KO Validated Antibodies 1

[KO Validated] CDK8 Rabbit Polyclonal Antibody (CAB5548)

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SKU:
CAB5548
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
K35, IDDHBA, K8
Research Use:
Epigenetics and Nuclear Signaling
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Description

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[KO Validated] CDK8 Antibody (CAB5548)

The [KO Validated] CDK8 Antibody (CAB5548) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is specifically designed for use in Western blot applications and is highly reactive with human samples.CDK8 is a member of the cyclin-dependent kinase (CDK) family and is involved in the regulation of gene expression by phosphorylating transcription factors. Dysregulation of CDK8 has been linked to various diseases, including cancer, making it an important target for therapeutic intervention.

This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] CDK8 Antibody
SKU:CAB5548
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:DDKG DKKN QQQQ QGNN HTNG TGHP GNQD SSHT QGPP LKKV RVVP PTTT SGGL IMTS DYQR SNPH AAYP NPGP STSQ PQSS MGYS ATSQ QPPQ YSHQ THRY
Tested Applications:WB IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:K35, IDDHBA, K8
Positive Sample:Jurkat
Cellular Localization:Nucleus.
Calculated MW:53kDa
Observed MW:56kDa

This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID1024
RRIDAB_2863508
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Jurkat cells, using [KO Validated] CDK8 Rabbit pAb (CAB5548) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageWestern blot analysis of lysates from wild type (WT) and CDK8 knockout (KO) HeLa cells, using [KO Validated] CDK8 Rabbit pAb (CAB5548) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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