KO Validated Antibodies 1

[KO Validated] HINT1 Rabbit Polyclonal Antibody (CAB10221)

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SKU:
CAB10221
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
HINT, NMAN, PKCI-1, PRKCNH1, HINT1
Research Use:
Cell Death
Frequently bought together:

Description

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[KO Validated] HINT1 Antibody (CAB10221)

The [KO Validated] HINT1 Antibody (CAB10221) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, has been rigorously tested for validation in knockout (KO) models, ensuring reliable and specific detection of HINT1 in experimental settings.With its high reactivity towards human samples, the CAB10221 antibody is ideally suited for use in Western blotting applications, allowing for the precise identification and quantification of HINT1 protein levels in different cell types and tissues.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] HINT1 Antibody
SKU:CAB10221
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MADE IAKA QVAR PGGD TIFG KIIR KEIP AKII FEDD RCLA FHDI SPQA PTHF LVIP KKHI SQIS VAED DDES LLGH LMIV GKKC AADL GLNK GYRM VVNE GSDG GQSV YHVH LHVL GGRQ MHWP PG
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:1000 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HINT, NMAN, PKCI-1, PRKCNH1, HINT1
Positive Sample:22Rv1, HeLa, H460, K-562, Mouse kidney, Mouse liver, Rat kidney, Rat liver
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:14kDa
Observed MW:14kDa

This gene encodes a protein that hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif. This gene is considered a tumor suppressor gene. In addition, mutations in this gene can cause autosomal recessive neuromyotonia and axonal neuropathy. There are several related pseudogenes on chromosome 7. Several transcript variants have been observed.

Purification MethodAffinity purification
Gene ID3094
RRIDAB_2757745
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using HINT1 Rabbit pAb (CAB10221) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunofluorescence analysis of H9C2 cells using HINT1 Rabbit pAb (CAB10221) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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