KO Validated Antibodies 1

[KO Validated] ERK1 Rabbit Polyclonal Antibody (CAB0228)

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SKU:
CAB0228
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK, K1
Research Use:
Cell Death
Frequently bought together:

Description

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[KO Validated] ERK1 Antibody (CAB0228)

The [KO Validated] ERK1 Antibody (CAB0228) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.MAPK3, also known as ERK1, is involved in various physiological and pathological processes, making it a valuable target for research in areas such as cancer, neurodegenerative diseases, and cardiovascular disorders.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] ERK1 Antibody
SKU:CAB0228
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAAA AAQG GGGG EPRR TEGV GPGV PGEV EMVK GQPF DVGP RYTQ LQYI GEGA YGMV SSAY DHVR KTRV AIKK ISPF EHQT YCQR TLRE IQIL LRFR HENV
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK, K1
Positive Sample:DU145, HeLa, 293T
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:43kDa
Observed MW:40kDa

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Purification MethodAffinity purification
Gene ID5595
RRIDAB_2757041
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KD Validated] ERK1 Rabbit pAb (CAB0228) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product ImageWestern blot analysis of lysates from wild type (WT) and ERK1 knockdown (KD) 293T cells, using [KD Validated] ERK1 Rabbit pAb (CAB0228) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human kidney using [KD Validated] ERK1 Rabbit pAb (CAB0228) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse heart using [KD Validated] ERK1 Rabbit pAb (CAB0228) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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