KO Validated Antibodies 1

[KO Validated] LC3B Rabbit Polyclonal Antibody (CAB7198)

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SKU:
CAB7198
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy
Reactivity:
Human, Mouse, Rat
Synonyms:
LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, 3B
Research Use:
Autophagy
Frequently bought together:

Description

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[KO Validated] LC3B Antibody (CAB7198)

The [KO Validated] LC3B Antibody (CAB7198) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity and sensitivity for detecting the MAP1LC3B protein in human samples, making it ideal for use in Western blot and immunofluorescence applications.MAP1LC3B, a key component of the autophagy pathway, is involved in the formation of autophagosomes which engulf and degrade cellular components, including damaged organelles and proteins. Dysregulation of autophagy has been linked to various diseases, including neurodegenerative disorders, cancer, and metabolic conditions.

This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] LC3B Antibody
SKU:CAB7198
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MPSE KTFK QRRT FEQR VEDV RLIR EQHP TKIP VIIE RYKG EKQL PVLD KTKF LVPD HVNM SELI KIIR RRLQ LNAN QAFF LLVN GHSM VSVS TPIS EVYE
Tested Applications:WB IHC-P ELISA IF-P
Recommended Dilution:
WB1:500 - 1:1000
IF-P1:50 - 1:200
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, 3B
Positive Sample:293T treated with Chloroquine, NIH/3T3 treated with Chloroquine, C6 treated with Chloroquine
Cellular Localization:Cytoplasm, Cytoplasmic Vesicle, Endomembrane System, Lipid-Anchor, Autophagosome, Autophagosome Membrane, Cytoskeleton.
Calculated MW:15kDa
Observed MW:14kDa/16kDa

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Purification MethodAffinity purification
Gene ID81631
RRIDAB_2863546
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KD Validated] LC3B Rabbit pAb (CAB7198) at 1:1000 dilution. NIH/3T3 and C6 cells were treated with Chloroquine (50 μM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageWestern blot analysis of lysates from wild type (WT) and LC3B knockdown (KD) 293T,293T+chloroquine cells, using [KD Validated] LC3B Rabbit pAb (CAB7198) at 1:1000 dilution. 293T cells were treated with Chloroquine (50 μM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human breast cancer using [KD Validated] LC3B Rabbit pAb (CAB7198) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human stomach using [KD Validated] LC3B Rabbit pAb (CAB7198) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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