Cell Cycle Antibodies 1

BUB3 Rabbit Polyclonal Antibody (CAB6536)

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SKU:
CAB6536
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
Reactivity:
Human, Mouse, Rat
Synonyms:
BUB3L, hBUB3, BUB3
Research Use:
Cell Cycle
Frequently bought together:

Description

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BUB3 Antibody (CAB6536)

The BUB3 Antibody (CAB6536) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blotting applications. By binding specifically to the BUB3 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for studies in cell biology and cancer research.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:BUB3 Antibody
SKU:CAB6536
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MTGS NEFK LNQP PEDG ISSV KFSP NTSQ FLLV SSWD TSVR LYDV PANS MRLK YQHT GAVL DCAF YDPT HAWS GGLD HQLK MHDL NTDQ ENLV GTHD APIR CVEY CPEV NVMV TGSW DQTV KLWD PRTP CNAG TFSQ PEKV YTLS VSGD RLIV GTAG RRVL VWDL RNMG YVQQ RRES SLKY QTRC IRAF PNKQ GYVL SSIE GRVA VEYL DPSP EVQK KKYA FKCH RLKE NNIE QIYP VNAI SFHN IHNT FATG GSDG FVNI WDPF NKKR LCQF HRYP TSIA SLAF SNDG TTLA IASS YMYE MDDT EHPE DGIF IRQV TDAE TKPK SPCT
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:BUB3L, hBUB3, BUB3
Positive Sample:MCF7, HL-60, HeLa, Mouse spleen, Mouse ovary, Mouse brain, Mouse lung
Cellular Localization:Chromosome, Nucleus, Centromere, Kinetochore.
Calculated MW:37kDa
Observed MW:40kDa

This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Purification MethodAffinity purification
Gene ID9184
RRIDAB_2767129
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using BUB3 Rabbit pAb (CAB6536) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
Product ImageImmunofluorescence analysis of U2OS cells using BUB3 Rabbit pAb (CAB6536).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution.
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