Epigenetics & Nuclear Signaling Antibodies 1

ATF6 Rabbit Polyclonal Antibody (CAB0202)

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SKU:
CAB0202
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
ACHM7, ATF6A, ATF6
Research Use:
Epigenetics and Nuclear Signaling
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Description

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ATF6 Antibody (CAB0202)

The ATF6 Antibody (CAB0202) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to ATF6, researchers can detect and analyze this important protein in a variety of cell types, making it ideal for studies in molecular biology and disease research.ATF6, also known as activating transcription factor 6, plays a crucial role in the cellular stress response by regulating the expression of genes involved in protein folding and quality control.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ATF6 Antibody
SKU:CAB0202
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:PKTQ TNSS VPAK TIII QTVP TLMP LAKQ QPII SLQP APTK GQTV LLSQ PTVV QLQA PGVL PSAQ PVLA VAGG VTQL PNHV VNVV PAPS ANSP VNGK LSVT KPVL QSTM RNV
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:5000
IF/ICC1:50 - 1:200
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ACHM7, ATF6A, ATF6
Positive Sample:Rat lung
Cellular Localization:Endoplasmic Reticulum Membrane, Nucleus, Single-Pass Type Ii Membrane Protein.
Calculated MW:75kDa
Observed MW:90-100kDa

This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis.

Purification MethodAffinity purification
Gene ID22926
RRIDAB_2757016
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Rat lung using ATF6 Rabbit pAb (CAB0202) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human kidney using ATF6 Rabbit pAb (CAB0202) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat colon using ATF6 Rabbit pAb (CAB0202) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using ATF6 Rabbit pAb (CAB0202) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of PC-12 cells using ATF6 Rabbit pAb (CAB0202) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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