Cell Cycle Antibodies 1

ARF6 Rabbit Polyclonal Antibody (CAB0988)

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SKU:
CAB0988
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
Reactivity:
Human, Mouse, Rat
Synonyms:
ARF6
Research Use:
Cell Cycle
Frequently bought together:

Description

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ARF6 Antibody (CAB0988)

The ARF6 Antibody (CAB0988) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it suitable for use in various research applications such as Western blotting.ARF6 is known for its role in regulating endocytic recycling and actin cytoskeleton dynamics, making it a key player in cell migration, invasion, and vesicular trafficking. Dysregulation of ARF6 has been implicated in cancer progression, making it a potential target for therapeutic interventions.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ARF6 Antibody
SKU:CAB0988
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MGKV LSKI FGNK EMRI LMLG LDAA GKTT ILYK LKLG QSVT TIPT VGFN VETV TYKN VKFN VWDV GGQD KIRP LWRH YYTG TQGL IFVV DCAD RDRI DEAR QELH RIIN DREM RDAI ILIF ANKQ DLPD AMKP HEIQ EKLG LTRI RDRN WYVQ PSCA TSGD GLYE GLTW LTSN YKS
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ARF6
Positive Sample:U-87MG, DU145, Mouse brain, Rat brain, Rat spleen
Cellular Localization:Cell Membrane, Cell Projection, Cleavage Furrow, Cytoplasm, Endosome Membrane, Golgi Apparatus, Lipid-Anchor, Midbody, Recycling Endosome Membrane, Filopodium Membrane.
Calculated MW:20kDa
Observed MW:19kDa

This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7.

Purification MethodAffinity purification
Gene ID382
RRIDAB_2757507
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using ARF6 Rabbit pAb (CAB0988) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 10s.
Product ImageImmunofluorescence analysis of HeLa cells using ARF6 Rabbit pAb (CAB0988) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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