The KDM1A Polyclonal Antibody (CAB21801) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.KDM1A, also known as Lysine-specific demethylase 1A, plays a crucial role in the epigenetic regulation of gene expression by removing methyl groups from lysine residues on histone proteins. This process impacts various cellular functions and pathways, making KDM1A a significant target for research in cancer, neurodegenerative diseases, and stem cell biology.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
KDM1A Polyclonal Antibody
SKU:
CAB21801
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AOF2, CPRF, KDM1, LSD1, BHC110, KDM1A
Positive Sample:
Mouse brain
Cellular Localization:
Nucleus.
Calculated MW:
93kDa
Observed MW:
110kDa
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
23028
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse brain, using KDM1A Rabbit pAb (CAB21801) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded Human kidney using KDM1A Rabbit pAb (CAB21801) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver using KDM1A Rabbit pAb (CAB21801) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Confocal immunofluorescence analysis of Hela cells using KDM1A Rabbit pAb (CAB21801) at dilution of 1:200. Blue: DAPI for nuclear staining.
