COL10A1 Polyclonal Antibody (CAB23884)

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SKU:
CAB23884
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
Synonyms:
COL10A1, collagen alpha-1(X) chain
Frequently bought together:

Description

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COL10A1 Polyclonal Antibody (CAB23884)

The COL10A1 Polyclonal Antibody (CAB23884) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the COL10A1 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential asset for studies in musculoskeletal biology and cartilage development.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:COL10A1 Polyclonal Antibody
SKU:CAB23884
Size:20μL, 100μL
Reactivity:Human
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MPEG FIKA GQRP SLSG TPLV SANQ GVTG MPVS AFTV ILSK AYPA IGTP IPFD KILY NRQQ HYDP RTGI FTCQ IPGI YYFS YHVH VKGT HVWV GLYK NGTP VMYT YDEY TKGY LDQA SGSA IIDL TEND QVWL QLPN AESN GLYS SEYV HSSF SGFL VAPM
Tested Applications:WB ELISA
Recommended Dilution:
WB1:100 - 1:500
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:COL10A1, collagen alpha-1(X) chain
Positive Sample:HT-1080
Cellular Localization:Secreted, Extracellular Matrix, Extracellular Space.
Calculated MW:66kDa
Observed MW:66kDa

This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD).

Purification MethodAffinity purification
Gene ID1300
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from HT-1080 cells, using COL10A1 Rabbit pAb (CAB23884) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
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