Human Cytokine Array (36 targets) (SARB0039)

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SKU:
SARB0039

Description

system_update_altDatasheet
Product Sku:SARB0039
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:36
Gene Symbols:BDNF, CCL11, CCL13, CCL18, CCL20, CCL23, CCL24, CCL26, CX3CL1, CXCL10|CXCL13, CXCL6, FGF4, FGF6, FGF7, FGF9, FLT3LG, GDNF, HGF, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IL16, LIF, MIF, NTF3, NTF4, PGF, PPBP, TGFB2, TGFB3, TIMP1, TIMP2, TNFRSF11B, TNFSF14
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
BDNF
  • Gene Symbol: BDNF
  • Gene ID: 627
  • Accession #: P23560
BLC (CXCL13)
  • Gene Symbol: CXCL13
  • Gene ID: 10563
  • Accession #: O43927
CK beta 8-1 (CCL23)
  • Gene Symbol: CCL23
  • Gene ID: 6368
  • Accession #: P55773
Eotaxin-1 (CCL11)
  • Gene Symbol: CCL11
  • Gene ID: 6356
  • Accession #: P51671
Eotaxin-2 (MPIF-2/CCL24)
  • Gene Symbol: Ccl24
  • Gene ID: 6369
  • Accession #: O00175
Eotaxin-3 (CCL26)
  • Gene Symbol: CCL26
  • Gene ID: 10344
  • Accession #: Q9Y258
FGF-4
  • Gene Symbol: FGF4
  • Gene ID: 2249
  • Accession #: P08620
FGF-6
  • Gene Symbol: FGF6
  • Gene ID: 2251
  • Accession #: P10767
FGF-7 (KGF)
  • Gene Symbol: FGF7
  • Gene ID: 2252
  • Accession #: P21781
FGF-9
  • Gene Symbol: FGF9
  • Gene ID: 2254
  • Accession #: P31371
Flt-3 Ligand
  • Gene Symbol: FLT3LG
  • Gene ID: 2323
  • Accession #: P49771
Fractalkine (CX3CL1)
  • Gene Symbol: CX3CL1
  • Gene ID: 6376
  • Accession #: P78423
GCP-2 (CXCL6)
  • Gene Symbol: CXCL6
  • Gene ID: 6372
  • Accession #: P80162
GDNF
  • Gene Symbol: GDNF
  • Gene ID: 2668
  • Accession #: P39905
HGF
  • Gene Symbol: HGF
  • Gene ID: 3082
  • Accession #: P14210
IGFBP-1
  • Gene Symbol: IGFBP1
  • Gene ID: 3484
  • Accession #: P08833
IGFBP-2
  • Gene Symbol: IGFBP2
  • Gene ID: 3485
  • Accession #: P18065
IGFBP-3
  • Gene Symbol: IGFBP3
  • Gene ID: 3486
  • Accession #: P17936
IGFBP-4
  • Gene Symbol: IGFBP4
  • Gene ID: 3487
  • Accession #: P22692
IL-16
  • Gene Symbol: IL16
  • Gene ID: 3603
  • Accession #: Q14005
LIF
  • Gene Symbol: LIF
  • Gene ID: 3976
  • Accession #: P15018
LIGHT (TNFSF14)
  • Gene Symbol: TNFSF14
  • Gene ID: 8740
  • Accession #: O43557
MCP-4 (CCL13)
  • Gene Symbol: CCL13
  • Gene ID: 6357
  • Accession #: Q99616
MIF
  • Gene Symbol: MIF
  • Gene ID: 4282
  • Accession #: P14174
NAP-2 (PPBP/CXCL7)
  • Gene Symbol: Ppbp
  • Gene ID: 5473
  • Accession #: P02775
NT-3
  • Gene Symbol: NTF3
  • Gene ID: 4908
  • Accession #: P20783
NT-4
  • Gene Symbol: NTF4
  • Gene ID: 4909
  • Accession #: P34130
Osteoprotegerin (TNFRSF11B)
  • Gene Symbol: TNFRSF11B
  • Gene ID: 4982
  • Accession #: O00300
PARC (CCL18)
  • Gene Symbol: CCL18
  • Gene ID: 6362
  • Accession #: P55774
PLGF
  • Gene Symbol: PGF
  • Gene ID: 5228
  • Accession #: P49763
TGF beta 2
  • Gene Symbol: TGFB2
  • Gene ID: 7042
  • Accession #: P61812
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TIMP-1
  • Gene Symbol: TIMP1
  • Gene ID: 7076
  • Accession #: P01033
TIMP-2
  • Gene Symbol: TIMP2
  • Gene ID: 7077
  • Accession #: P16035
MIP-3 alpha (CCL20)
  • Gene Symbol: CCL20
  • Gene ID: 6364
  • Accession #: P78556
IP-10 (CXCL10)
  • Gene Symbol: CXCL10
  • Gene ID: 3627
  • Accession #: P02778
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Cytokine Antibody Array C4 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
BDNF
Analytes:
BLC (CXCL13)
Analytes:
CK beta 8-1 (CCL23)
Analytes:
Eotaxin-1 (CCL11)
Analytes:
Eotaxin-2 (MPIF-2/CCL24)
Analytes:
Eotaxin-3 (CCL26)
Analytes:
FGF-4
Analytes:
FGF-6
Analytes:
FGF-7 (KGF)
Analytes:
FGF-9
Analytes:
Flt-3 Ligand
Analytes:
Fractalkine (CX3CL1)
Analytes:
GCP-2 (CXCL6)
Analytes:
GDNF
Analytes:
HGF
Analytes:
IGFBP-1
Analytes:
IGFBP-2
Analytes:
IGFBP-3
Analytes:
IGFBP-4
Analytes:
IL-16
Analytes:
LIF
Analytes:
LIGHT (TNFSF14)
Analytes:
MCP-4 (CCL13)
Analytes:
MIF
Analytes:
NAP-2 (PPBP/CXCL7)
Analytes:
NT-3
Analytes:
NT-4
Analytes:
Osteoprotegerin (TNFRSF11B)
Analytes:
PARC (CCL18)
Analytes:
PLGF
Analytes:
TGF beta 2
Analytes:
TGF beta 3
Analytes:
TIMP-1
Analytes:
TIMP-2
Analytes:
MIP-3 alpha (CCL20)
Analytes:
IP-10 (CXCL10)
Reactivity:
Human
Applications:
Multiplex Array
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