Human Cytokine Array (54 targets) (SARB0043)

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SKU:
SARB0043

Description

system_update_altDatasheet
Product Sku:SARB0043
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:54
Gene Symbols:ALCAM, BMP5, BMP7, CCL23, CD14, CD80, CDH5, CSF1R, CTF1, CXCL10|CXCL12, CXCL16, ENG, ERBB3, FASLG, FLT4, ICAM2, IGF2, IL10RB, IL13RA2, IL18BP, IL18RAP, IL1R2, IL21R, IL2RB, IL2RG, IL5RA, IL9, INHBA, KDR, KIT, LEPR, LIF, MMP1, MMP13, MMP3, MMP9, NGFR, PDGFA, PDGFB, PDGFRA, PDGFRB, PECAM1, PRL, SELE, SELL, SIGLEC5, TEK, TGFA, TGFB1, TGFB2, TIE1, TIMP4, TNFRSF21
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Activin A
  • Gene Symbol: INHBA
  • Gene ID: 3624
  • Accession #: P08476
ALCAM (CD166)
  • Gene Symbol: ALCAM
  • Gene ID: 214
  • Accession #: Q13740
CD80 (B7-1)
  • Gene Symbol: CD80
  • Gene ID: 941
  • Accession #: P33681
BMP-5
  • Gene Symbol: BMP5
  • Gene ID: 653
  • Accession #: P22003
BMP-7
  • Gene Symbol: BMP7
  • Gene ID: 655
  • Accession #: P18075
Cardiotrophin-1 (CT-1)
  • Gene Symbol: CTF1
  • Gene ID: 1489
  • Accession #: Q16619
CD14
  • Gene Symbol: CD14
  • Gene ID: 929
  • Accession #: P08571
CXCL16
  • Gene Symbol: CXCL16
  • Gene ID: 58191
  • Accession #: Q9H2A7
DR6 (TNFRSF21)
  • Gene Symbol: TNFRSF21
  • Gene ID: 27242
  • Accession #: O75509
Endoglin (CD105)
  • Gene Symbol: ENG
  • Gene ID: 2022
  • Accession #: P17813
ErbB3
  • Gene Symbol: ErbB3
  • Gene ID: 2065
  • Accession #: P21860
E-Selectin
  • Gene Symbol: SELE
  • Gene ID: 6401
  • Accession #: P16581
Fas Ligand (TNFSF6)
  • Gene Symbol: FASLG
  • Gene ID: 356
  • Accession #: P48023
ICAM-2 (CD102)
  • Gene Symbol: ICAM2
  • Gene ID: 3384
  • Accession #: P13598
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
IL-1 R2
  • Gene Symbol: IL1R2
  • Gene ID: 7850
  • Accession #: P27930
IL-10 R beta
  • Gene Symbol: IL10RB
  • Gene ID: 3588
  • Accession #: Q08334
IL-13 R alpha 2
  • Gene Symbol: IL13RA2
  • Gene ID: 3598
  • Accession #: Q14627
IL-18 BP alpha
  • Gene Symbol: IL18BP
  • Gene ID: 10068
  • Accession #: O95998
IL-18 R beta (AcPL)
  • Gene Symbol: IL18RAP
  • Gene ID: 8807
  • Accession #: O95256
MMP-3
  • Gene Symbol: MMP3
  • Gene ID: 4314
  • Accession #: P08254
IL-2 R beta (CD122)
  • Gene Symbol: IL2RB
  • Gene ID: 3560
  • Accession #: P14784
IL-2 R gamma (Common gamma Chain)
  • Gene Symbol: IL2RG
  • Gene ID: 3561
  • Accession #: P31785
IL-21 R
  • Gene Symbol: IL21R
  • Gene ID: 50615
  • Accession #: Q9HBE5
IL-5 R alpha
  • Gene Symbol: IL5RA
  • Gene ID: 3568
  • Accession #: Q01344
IL-9
  • Gene Symbol: IL9
  • Gene ID: 3578
  • Accession #: P15248
IP-10 (CXCL10)
  • Gene Symbol: CXCL10
  • Gene ID: 3627
  • Accession #: P02778
LAP/TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
Leptin R
  • Gene Symbol: LEPR
  • Gene ID: 3953
  • Accession #: P48357
LIF
  • Gene Symbol: LIF
  • Gene ID: 3976
  • Accession #: P15018
L-Selectin (CD62L)
  • Gene Symbol: SELL
  • Gene ID: 6402
  • Accession #: P14151
M-CSF R
  • Gene Symbol: CSF1R
  • Gene ID: 1436
  • Accession #: P07333
MMP-1
  • Gene Symbol: MMP1
  • Gene ID: 4312
  • Accession #: P03956
MMP-13
  • Gene Symbol: MMP13
  • Gene ID: 4322
  • Accession #: P45452
MMP-9
  • Gene Symbol: MMP9
  • Gene ID: 4318
  • Accession #: P14780
MPIF-1 (CCL23)
  • Gene Symbol: CCL23
  • Gene ID: 6368
  • Accession #: P55773
NGFR (TNFRSF16)
  • Gene Symbol: NGFR
  • Gene ID: 4804
  • Accession #: P08138
PDGF-AA
  • Gene Symbol: PDGFA
  • Gene ID: 5154
  • Accession #: P04085
PDGF-AB
  • Gene Symbol: PDGFB
  • Gene ID: 5155
  • Accession #: P01127
PDGF R alpha
  • Gene Symbol: PDGFRA
  • Gene ID: 5156
  • Accession #: P16234
PDGF R beta
  • Gene Symbol: PDGFRB
  • Gene ID: 5159
  • Accession #: P09619
PECAM-1 (CD31)
  • Gene Symbol: PECAM1
  • Gene ID: 5175
  • Accession #: P16284
Prolactin
  • Gene Symbol: PRL
  • Gene ID: 5617
  • Accession #: P01236
SCF R (CD117/c-kit)
  • Gene Symbol: KIT
  • Gene ID: 3815
  • Accession #: P10721
SDF-1 beta (CXCL12 beta)
  • Gene Symbol: CXCL12
  • Gene ID: 6387
  • Accession #: P48061
Siglec-5 (CD170)
  • Gene Symbol: SIGLEC5
  • Gene ID: 8778
  • Accession #: O15389
TGF alpha
  • Gene Symbol: TGFA
  • Gene ID: 7039
  • Accession #: P01135
TGF beta 2
  • Gene Symbol: TGFB2
  • Gene ID: 7042
  • Accession #: P61812
Tie-1
  • Gene Symbol: TIE1
  • Gene ID: 7075
  • Accession #: P35590
Tie-2
  • Gene Symbol: TEK
  • Gene ID: 7010
  • Accession #: Q02763
TIMP-4
  • Gene Symbol: TIMP4
  • Gene ID: 7079
  • Accession #: Q99727
VE-Cadherin (CDH5)
  • Gene Symbol: CDH5
  • Gene ID: 1003
  • Accession #: P33151
VEGFR2
  • Gene Symbol: KDR
  • Gene ID: 3791
  • Accession #: P35968
VEGFR3
  • Gene Symbol: FLT4
  • Gene ID: 2324
  • Accession #: P35916
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Cytokine Antibody Array C8 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
Activin A
Analytes:
ALCAM (CD166)
Analytes:
CD80 (B7-1)
Analytes:
BMP-5
Analytes:
BMP-7
Analytes:
Cardiotrophin-1 (CT-1)
Analytes:
CD14
Analytes:
CXCL16
Analytes:
DR6 (TNFRSF21)
Analytes:
Endoglin (CD105)
Analytes:
ErbB3
Analytes:
E-Selectin
Analytes:
Fas Ligand (TNFSF6)
Analytes:
ICAM-2 (CD102)
Analytes:
IGF-2
Analytes:
IL-1 R2
Analytes:
IL-10 R beta
Analytes:
IL-13 R alpha 2
Analytes:
IL-18 BP alpha
Analytes:
IL-18 R beta (AcPL)
Analytes:
MMP-3
Analytes:
IL-2 R beta (CD122)
Analytes:
IL-2 R gamma (Common gamma Chain)
Analytes:
IL-21 R
Analytes:
IL-5 R alpha
Analytes:
IL-9
Analytes:
IP-10 (CXCL10)
Analytes:
LAP/TGF beta 1
Analytes:
Leptin R
Analytes:
LIF
Analytes:
L-Selectin (CD62L)
Analytes:
M-CSF R
Analytes:
MMP-1
Analytes:
MMP-13
Analytes:
MMP-9
Analytes:
MPIF-1 (CCL23)
Analytes:
NGFR (TNFRSF16)
Analytes:
PDGF-AA
Analytes:
PDGF-AB
Analytes:
PDGF R alpha
Analytes:
PDGF R beta
Analytes:
PECAM-1 (CD31)
Analytes:
Prolactin
Analytes:
SCF R (CD117/c-kit)
Reactivity:
Human
Applications:
Multiplex Array
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