Human Cytokine Array (32 targets) (SARB0035)

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SKU:
SARB0035

Description

system_update_altDatasheet
Product Sku:SARB0035
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:32
Gene Symbols:AHSG, ALB, AMICA1, ANGPT4, CD163, CLU, CRTAM, CSF3R, CST3, CXCL14|DLL1, FGF1, FOLR1, GPRASP1, GPRASP2, IL17RB, IL27, LAG3, LDLR, PGA3, PGA4, PGA5, PRAP1, RBP4, SDC1, SOST, SPINT2, TFPI, THBS1, TNFRSF13B, TNFSF11, TNFSF13B, TNNI2, WISP1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
aFGF (FGF-1)
  • Gene Symbol: FGF1
  • Gene ID: 2246
  • Accession #: P05230
Albumin
  • Gene Symbol: ALB
  • Gene ID: 213
  • Accession #: P02768
AMICA
  • Gene Symbol: AMICA1
  • Gene ID: 120425
  • Accession #: Q86YT9
Angiopoietin-4
  • Gene Symbol: ANGPT4
  • Gene ID: 51378
  • Accession #: Q9Y264
BAFF
  • Gene Symbol: TNFSF13B
  • Gene ID: 10673
  • Accession #: Q9Y275
CD163
  • Gene Symbol: CD163
  • Gene ID: 9332
  • Accession #: Q86VB7
Clusterin
  • Gene Symbol: CLU
  • Gene ID: 1191
  • Accession #: P10909
CRTAM
  • Gene Symbol: CRTAM
  • Gene ID: 56253
  • Accession #: O95727
CXCL14 (BRAK)
  • Gene Symbol: CXCL14
  • Gene ID: 9547
  • Accession #: O95715
Cystatin C
  • Gene Symbol: CST3
  • Gene ID: 1471
  • Accession #: P01034
DLL1
  • Gene Symbol: DLL1
  • Gene ID: 28514
  • Accession #: O00548
Fetuin A
  • Gene Symbol: AHSG
  • Gene ID: 197
  • Accession #: P02765
FOLR1
  • Gene Symbol: FOLR1
  • Gene ID: 2348
  • Accession #: P15328
GASP-1 (WFIKKNRP)
  • Gene Symbol: GPRASP1
  • Gene ID: 9737
  • Accession #: Q5JY77
GASP-2 (WFIKKN)
  • Gene Symbol: GPRASP2
  • Gene ID: 114928
  • Accession #: Q96D09
GCSF R (CD114)
  • Gene Symbol: CSF3R
  • Gene ID: 1441
  • Accession #: Q99062
HAI-2
  • Gene Symbol: SPINT2
  • Gene ID: 10653
  • Accession #: O43291
IL-17 RB
  • Gene Symbol: IL17RB
  • Gene ID: 55540
  • Accession #: Q9NRM6
IL-27
  • Gene Symbol: IL27
  • Gene ID: 246778
  • Accession #: Q8NEV9
LAG-3
  • Gene Symbol: LAG3
  • Gene ID: 3902
  • Accession #: P18627
LDL R
  • Gene Symbol: LDLR
  • Gene ID: 3949
  • Accession #: P01130
Pepsinogen 1
  • Gene Symbol: PGA3/PGA4/PGA5
  • Gene ID: N/A
  • Accession #: N/A
RBP4
  • Gene Symbol: RBP4
  • Gene ID: 5950
  • Accession #: P02753
SOST
  • Gene Symbol: SOST
  • Gene ID: 50964
  • Accession #: Q9BQB4
Syndecan-1
  • Gene Symbol: SDC1
  • Gene ID: 6382
  • Accession #: P18827
TACI (TNFRSF13B)
  • Gene Symbol: TNFRSF13B
  • Gene ID: 23495
  • Accession #: O14836
TFPI
  • Gene Symbol: TFPI
  • Gene ID: 7035
  • Accession #: P10646
Thrombospondin 1
  • Gene Symbol: THBS1
  • Gene ID: 7057
  • Accession #: P07996
TRANCE (TNFSF11)
  • Gene Symbol: TNFSF11
  • Gene ID: 8600
  • Accession #: O14788
Troponin I
  • Gene Symbol: TNNI2
  • Gene ID: 7136
  • Accession #: P48788
uPA
  • Gene Symbol: PRAP1
  • Gene ID: 118471
  • Accession #: Q96NZ9
WISP-1 (CCN4)
  • Gene Symbol: WISP1
  • Gene ID: 8840
  • Accession #: O95388
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Cytokine Antibody Array C12 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
aFGF (FGF-1)
Analytes:
Albumin
Analytes:
AMICA
Analytes:
Angiopoietin-4
Analytes:
BAFF
Analytes:
CD163
Analytes:
Clusterin
Analytes:
CRTAM
Analytes:
CXCL14 (BRAK)
Analytes:
Cystatin C
Analytes:
DLL1
Analytes:
Fetuin A
Analytes:
FOLR1
Analytes:
GASP-1 (WFIKKNRP)
Analytes:
GASP-2 (WFIKKN)
Analytes:
GCSF R (CD114)
Analytes:
HAI-2
Analytes:
IL-17 RB
Analytes:
IL-27
Analytes:
LAG-3
Analytes:
LDL R
Analytes:
Pepsinogen 1
Analytes:
RBP4
Analytes:
SOST
Analytes:
Syndecan-1
Analytes:
TACI (TNFRSF13B)
Analytes:
TFPI
Analytes:
Thrombospondin 1
Analytes:
TRANCE (TNFSF11)
Analytes:
Troponin I
Analytes:
uPA
Analytes:
WISP-1 (CCN4)
Reactivity:
Human
Applications:
Multiplex Array
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