Antibody Name:	Anti-ZBTB33 Antibody
Antibody SKU:	CAB12900
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 523-672 of human ZBTB33 (NP_006768.1).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, Mouse kidney, Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 523-672 of human ZBTB33 (NP_006768.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PCRY CEKV FPLA EYRT KHEI HHTG ERRY QCLA CGKS FINY QFMS SHIK SVHS QDPS GDSK LYRL HPCR SLQI RQYA YLSD RSST IPAM KDDG IGYK VDTG KEPP VGTT TSTQ NKPM TWED IFIQ QEND SIFK QNVT DGST EFEF IIPE SY
Gene ID:	10009
Uniprot:	Q86T24
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	74kDa
Observed MW:	74-90kDa

Synonyms:

ZBTB33, ZNF-kaiso, ZNF348

Background:

This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-alpha2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.

UniProt Protein Function:	Kaiso: Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.
UniProt Protein Details:	Protein type:C2H2-type zinc finger protein; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: Xq24 Cellular Component: cytosol; nucleolus; nucleoplasm; nucleus; plasma membrane Molecular Function:methyl-CpG binding; protein binding; sequence-specific DNA binding Biological Process: negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, May 2010]
UniProt Code:	Q86T24
NCBI GenInfo Identifier:	84029319
NCBI Gene ID:	10009
NCBI Accession:	Q86T24.2
UniProt Secondary Accession:	Q86T24,O00319, Q7Z361, Q8IVP6, Q8N3P0, B2R5U6,
UniProt Related Accession:	Q86T24
Molecular Weight:	74kDa
NCBI Full Name:	Transcriptional regulator Kaiso
NCBI Synonym Full Names:	zinc finger and BTB domain containing 33
NCBI Official Symbol:	ZBTB33
NCBI Official Synonym Symbols:	ZNF348; ZNF-kaiso
NCBI Protein Information:	transcriptional regulator Kaiso
UniProt Protein Name:	Transcriptional regulator Kaiso
UniProt Synonym Protein Names:	Zinc finger and BTB domain-containing protein 33
Protein Family:	Transcriptional regulator
UniProt Gene Name:	ZBTB33

	Western blot analysis of extracts of various cell lines, using Kaiso/Kaiso/ZBTB33 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
	Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Kaiso/ZBTB33 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.