Cell Biology Antibodies 14

WIPF1 Rabbit Polyclonal Antibody (CAB17003)

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SKU:
CAB17003
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
WIP, WAS2, PRPL-2, WASPIP, WIPF1
Research Use:
Cell Biology
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Description

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WIPF1 Antibody (CAB17003)

The WIPF1 Antibody (CAB17003) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific and sensitive to human samples, making it ideal for Western blot applications. By binding to the WIPF1 protein, this antibody allows for the detection and analysis of WIPF1 in various cell types, essential for studies in cell biology, cancer research, and immunology.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:WIPF1 Antibody
SKU:CAB17003
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:QLPS RSGV DSPR SGPR PPLP PDRP SAGA PPPP PPST SIRN GFQD SPCE DEWE SRFY FHPI SDLP PPEP YVQT TKSY PSKL ARNE SRSG SNRR ERGA PPLP P
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:100 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:WIP, WAS2, PRPL-2, WASPIP, WIPF1
Positive Sample:RAW264.7, Mouse spleen
Cellular Localization:Actin Cytoskeleton, Cytoplasmic Vesicle, Cytosol.
Calculated MW:51kDa
Observed MW:51kDa

This gene encodes a protein that plays an important role in the organization of the actin cytoskeleton. The encoded protein binds to a region of Wiskott-Aldrich syndrome protein that is frequently mutated in Wiskott-Aldrich syndrome, an X-linked recessive disorder. Impairment of the interaction between these two proteins may contribute to the disease. Two transcript variants encoding the same protein have been identified for this gene.

Purification MethodAffinity purification
Gene ID7456
RRIDAB_2772897
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using WIPF1 Rabbit pAb (CAB17003) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human appendix using WIPF1 Rabbit pAb (CAB17003) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse spleen using WIPF1 Rabbit pAb (CAB17003) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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