Cell Biology Antibodies 12

VPS28 Rabbit Polyclonal Antibody (CAB9104)

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SKU:
CAB9104
Product Type:
Antibody
Applications:
WB
Applications:
IF
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
VPS28
Research Use:
Cell Biology
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Description

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VPS28 Antibody (CAB9104)

The VPS28 Antibody (CAB9104) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications.VPS28 plays a crucial role in the sorting of ubiquitinated cargo proteins and is essential for multivesicular body (MVB) biogenesis and lysosomal degradation. Dysregulation of VPS28 has been linked to various diseases, including neurodegenerative disorders and viral infections, underscoring its importance in cellular homeostasis.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:VPS28 Antibody
SKU:CAB9104
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MFHG IPAT PGIG APGN KPEL YEEV KLYK NARE REKY DNMA ELFA VVKT MQAL EKAY IKDC VSPS EYTA ACSR LLVQ YKAA FRQV QGSE ISSI DEFC RKFR LDCP LAME RIKE DRPI TIKD DKGN LNRC IADV VSLF ITVM DKLR LEIR AMDE IQPD LREL METM HRMS HLPP DFEG RQTV SQWL QTLS GMSA SDEL DDSQ VRQM LFDL ESAY NAFN RFLH A
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:VPS28
Positive Sample:A-549, U-251MG, 293T, HepG2, Mouse brain, Mouse kidney, Mouse liver, Mouse spleen, Rat brain
Cellular Localization:Cell Membrane, Late Endosome Membrane, Peripheral Membrane Protein.
Calculated MW:25kDa
Observed MW:25kDa

This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms.

Purification MethodAffinity purification
Gene ID51160
RRIDAB_2772868
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using VPS28 Rabbit pAb (CAB9104) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
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