Signal Transduction Antibodies 2

USP5 Rabbit Polyclonal Antibody (CAB4202)

(No reviews yet) Write a Review
SKU:
CAB4202
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
ISOT, USP5
Research Use:
Signal Transduction
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

USP5 Antibody (CAB4202)

The USP5 Antibody (CAB4202) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-derived antibody has high specificity for human samples and is validated for use in Western blotting applications. By binding to the USP5 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in the fields of molecular biology, biochemistry, and cancer research.USP5, also known as ubiquitin-specific protease 5, plays a crucial role in maintaining protein homeostasis by regulating the degradation of ubiquitinated proteins. Its functions in the ubiquitin-proteasome system have implications in various cellular processes, including DNA repair, cell cycle progression, and apoptosis.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:USP5 Antibody
SKU:CAB4202
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAEL SEEA LLSV LPTI RVPK AGDR VHKD ECAF SFDT PESE GGLY ICMN TFLG FGKQ YVER HFNK TGQR VYLH LRRT RRPK EEDP ATGT GDPP RKKP TRLA IGVE GGFD LSEE KFEL DEDV KIVI LPDY LEIA RDGL GGLP DIVR DRVT SAVE ALLS ADSA SRKQ EVQA WDGE VRQV SKHA FSLK QLDN PARI PPCG WKCS KCDM RENL WLNL TDGS ILCG RRYF DGSG GNNH AVEH YRET GYPL AVKL GTIT PDGA DVYS YDED DMVL DPSL AEHL SHFG
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:1000 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ISOT, USP5
Positive Sample:LO2, A-549, 293T, HeLa, Jurkat, 22Rv1, Mouse kidney, Mouse brain
Cellular Localization:Cytosol, Lysosome, Nucleus.
Calculated MW:96kDa
Observed MW:110kDa

Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).

Purification MethodAffinity purification
Gene ID8078
RRIDAB_2765553
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using USP5 Rabbit pAb (CAB4202) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat stomach using USP5 Rabbit pAb (CAB4202) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human breast cancer using USP5 Rabbit pAb (CAB4202) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse kidney using USP5 Rabbit pAb (CAB4202) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of L929 cells using USP5 Rabbit pAb (CAB4202) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products