Metabolism Antibodies 1

UMPS Rabbit Polyclonal Antibody (CAB13251)

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SKU:
CAB13251
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Reactivity:
Human, Mouse, Rat
Synonyms:
OPRT, UMPS
Research Use:
Metabolism
Frequently bought together:

Description

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UMPS Antibody (CAB13251)

The UMPS Antibody (CAB13251) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the UMPS protein, this antibody enables precise detection and analysis in various cell types, making it an ideal choice for studies in fields such as biochemistry and molecular biology.UMPS is an essential enzyme involved in the de novo biosynthesis of pyrimidine nucleotides, vital for DNA and RNA synthesis in cells.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:UMPS Antibody
SKU:CAB13251
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:TKDY GTKR LVEG TINP GETC LIIE DVVT SGSS VLET VEVL QKEG LKVT DAIV LLDR EQGG KDKL QAHG IRLH SVCT LSKM LEIL EQQK KVDA ETVG RVKR FIQE NVFV AANH NGSP LSIK EAPK ELSF GARA ELPR IHPV ASKL LRLM QKKE TNLC LSAD V
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:OPRT, UMPS
Positive Sample:HT-29, U-87MG, HeLa, LO2, Mouse brain, Mouse kidney
Cellular Localization:Cytoplasm, Cytosol, Nucleus.
Calculated MW:52kDa
Observed MW:57kDa

This gene encodes a uridine 5'-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5'-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5'-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID7372
RRIDAB_2760104
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using UMPS Rabbit pAb (CAB13251) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunofluorescence analysis of NIH-3T3 cells using UMPS Rabbit pAb (CAB13251) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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