Anti-TROP-2 Antibody (CAB20824)
- SKU:
- CAB20824
- Product Type:
- Antibody
- Antibody Type:
- Secondary Antibody
- Host Species:
- Goat
- Isotype:
- IgG
Description
| Antibody Name: | Anti-TROP-2 Antibody (CAB20824) |
| Antibody SKU: | CAB20824 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunohistochemistry, ImmunofluorescenceFlow Cytometry |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 27-274 of human TROP-2 (NP_002344.2). |
| Application: | Western blotting, Immunohistochemistry, ImmunofluorescenceFlow Cytometry |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 FC 1:100 - 1:800 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, A-431, Mouse testis, Mouse kidney, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 27-274 of human TROP-2 (NP_002344.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HTAA QDNC TCPT NKMT VCSP DGPG GRCQ CRAL GSGM AVDC STLT SKCL LLKA RMSA PKNA RTLV RPSE HALV DNDG LYDP DCDP EGRF KARQ CNQT SVCW CVNS VGVR RTDK GDLS LRCD ELVR THHI LIDL RHRP TAGA FNHS DLDA ELRR LFRE RYRL HPKF VAAV HYEQ PTIQ IELR QNTS QKAA GDVD IGDA AYYF ERDI KGES LFQG RGGL DLRV RGEP LQVE RTLI YYLD EIPP KFSM KRLT |
| Cellular Location: | Membrane, Single-pass type I membrane protein |
| Calculated MW: | 35kDa |
| Observed MW: | 40-65KDa |
| Synonyms: | TACSTD2, EGP-1, EGP1, GA733-1, GA7331, GP50, M1S1, TROP2 |
| Background: | This intronless gene encodes a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of this gene have been associated with gelatinous drop-like corneal dystrophy. |
 | Immunohistochemistry of paraffin-embedded human placenta using TROP-2 Rabbit mAb at dilution of 1:8100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human tonsil using TROP-2 Rabbit mAb at dilution of 1:8100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded Human liver using TROP-2 antibody at dilution of 1:2700 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human lung squamous carcinoma tissue using TROP-2 Rabbit mAb at dilution of 1:8100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded Human placenta using TROP-2 antibody at dilution of 1:2700 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded Human lung squamous carcinoma tissue using TROP-2 antibody at dilution of 1:2700 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded Human tonsil using TROP-2 antibody at dilution of 1:2700 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Western blot analysis of extracts of various cell lines, using TROP-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s. |
 | Western blot analysis of extracts of various cell lines, using TROP-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |
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