Epigenetics & Nuclear Signaling Antibodies 4

Argonaute-2 Rabbit Polyclonal Antibody (CAB6802)

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SKU:
CAB6802
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
PPD, Q10, CASC7, EIF2C2, LESKRES, LINC00980, Argonaute-2
Research Use:
Epigenetics and Nuclear Signaling
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Description

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Argonaute-2 Antibody (CAB6802)

The Argonaute-2 Antibody (CAB6802) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity for EIF2C2 in human samples and is optimized for Western blot applications. By binding to EIF2C2, this antibody enables precise detection and analysis of the protein in various cell types, making it an essential component for research in molecular biology and gene regulation.EIF2C2, also known as Argonaute 2, plays a crucial role in RNA silencing and post-transcriptional gene regulation.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Argonaute-2 Antibody
SKU:CAB6802
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MDIP KIDI YHYE LDIK PEKC PRRV NREI VEHM VQHF KTQI FGDR KPVF DGRK NLYT AMPL PIGR DKVE L
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PPD, Q10, CASC7, EIF2C2, LESKRES, LINC00980, Argonaute-2
Positive Sample:Jurkat, 293T, HeLa
Cellular Localization:Cytoplasm, Nucleus, P-Body.
Calculated MW:97kDa
Observed MW:110kDa

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID27161
RRIDAB_2767383
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Argonaute-2 Rabbit pAb (CAB6802) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Product ImageWestern blot analysis of various lysates, using Argonaute-2 Rabbit pAb (CAB6802) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021) Exposure time: 60s.
Product ImageImmunofluorescence analysis of HeLa cells using Argonaute-2 Rabbit pAb (CAB6802) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of HepG2 cells using Argonaute-2 Rabbit pAb (CAB6802) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of PC-12 cells using Argonaute-2 Rabbit pAb (CAB6802) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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