Cell Biology Antibodies 8

TRAP1 Rabbit Polyclonal Antibody (CAB2748)

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SKU:
CAB2748
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
HSP75, HSP 75, HSP90L, TRAP-1, TRAP1
Research Use:
Cell Biology
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Description

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TRAP1 Antibody (CAB2748)

The TRAP1 Antibody (CAB2748) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting TRAP1 in human samples, making it ideal for use in Western blot applications.TRAP1, also known as Tumor necrosis factor receptor-associated protein 1, is a key player in maintaining mitochondrial homeostasis and protecting cells from stress-induced damage.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:TRAP1 Antibody
SKU:CAB2748
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:GLFM REGI VTAT EQEV KEDI AKLL RYES SALP SGQL TSLS EYAS RMRA GTRN IYYL CAPN RHLA EHSP YYEA MKKK DTEV LFCF EQFD ELTL LHLR EFDK KKLI SVET DIVV DHYK EEKF EDRS PAAE CLSE KETE ELMA WMRN VLGS RVTN VKVT LRLD THPA MVTV LEMG AARH FLRM QQLA KTQE ERAQ LLQP TLEI NPRH ALIK KLNQ LRAS EPGL AQLL VDQI YENA MIAA GLVD DPRA MVGR LNEL LVKA LERH
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HSP75, HSP 75, HSP90L, TRAP-1, TRAP1
Positive Sample:HeLa, HCT 116, NIH/3T3
Cellular Localization:Mitochondrion, Mitochondrion Inner Membrane, Mitochondrion Matrix.
Calculated MW:80kDa
Observed MW:71kDa

This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID10131
RRIDAB_2764597
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using TRAP1 Rabbit pAb (CAB2748) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells, using TRAP1 Rabbit pAb (CAB2748) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma using TRAP1 Rabbit pAb (CAB2748) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse heart using TRAP1 Rabbit pAb (CAB2748) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of HeLa cells using TRAP1 Rabbit pAb (CAB2748) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of HepG2 cells using TRAP1 Rabbit pAb (CAB2748) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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