Cell Biology Antibodies 18

TPX2 Rabbit Polyclonal Antibody (CAB18327)

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SKU:
CAB18327
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Rat
Synonyms:
DIL2, p100, DIL-2, HCTP4, FLS353, HCA519, REPP86, C20orf1, C20orf2, GD:C20orf1, TPX2
Research Use:
Cell Biology
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Description

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TPX2 Antibody (CAB18327)

The TPX2 Antibody (CAB18327) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is specifically optimized for use in various applications, including Western blot, immunofluorescence, immunoprecipitation, and flow cytometry.TPX2 is a well-known regulator of microtubule organization and plays a crucial role in ensuring proper chromosome segregation during cell division. Dysregulation of TPX2 expression has been linked to cancer development and progression, making it a promising target for cancer research and drug development.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Rat samples.

Product Name:TPX2 Antibody
SKU:CAB18327
Size:20μL, 100μL
Reactivity:Human, Rat
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSQV KSSY SYDA PSDF INFS SLDD EGDT QNID SWFE EKAN LENK LLGK NGTG GLFQ GKTP LRKA NLQQ AIVT PLKP VDNT YYKE AEKE NLVE QSIP SNAC SSLE VEAA ISRK TPAQ PQRR SLRL SAQK DLEQ KEKH HVKM KAKR CATP VIID EILP SKKM KVSN NKKK PEEE GSAH QDTA EKNA SSPE KAKG RHTV PCMP PAKQ KFLK STEE QELE KSMK MQQE VVEM RKKN EEFK KLAL AGIG QPVK KS
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:DIL2, p100, DIL-2, HCTP4, FLS353, HCA519, REPP86, C20orf1, C20orf2, GD:C20orf1, TPX2
Positive Sample:A-549
Cellular Localization:Cytoplasm, Nucleus, Cytoskeleton, Spindle, Spindle Pole.
Calculated MW:86kDa
Observed MW:105kDa

Enables importin-alpha family protein binding activity and protein kinase binding activity. Involved in activation of protein kinase activity; microtubule cytoskeleton organization; and negative regulation of microtubule depolymerization. Located in intercellular bridge; mitotic spindle; and nucleoplasm. Colocalizes with spindle pole.

Purification MethodAffinity purification
Gene ID22974
RRIDAB_2862099
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from A-549 cells, using TPX2 Rabbit pAb (CAB18327) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma using TPX2 Rabbit pAb (CAB18327) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells using TPX2 Rabbit pAb (CAB18327) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using TPX2 Rabbit pAb (CAB18327) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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