Name: Anti-SUZ12 Antibody (CAB7786)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB7786
Availability: OutOfStock

Antibody Name:	Anti-SUZ12 Antibody [KO Validated]
Antibody SKU:	CAB7786
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 470-739 of human SUZ12 (NP_056170.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, BT-474, MCF-7, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 470-739 of human SUZ12 (NP_056170.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CHSR FIFN YVYH PKGA RIDV SINE CYDG SYAG NPQD IHRQ PGFA FSRN GPVK RTPI THIL VCRP KRTK ASMS EFLE SEDG EVEQ QRTY SSGH NRLY FHSD TCLP LRPQ EMEV DSED EKDP EWLR EKTI TQIE EFSD VNEG EKEV MKLW NLHV MKHG FIAD NQMN HACM LFVE NYGQ KIIK KNLC RNFM LHLV SMHD FNLI SIMS IDKA VTKL REMQ QKLE KGES ASPA NEEI TEEQ NGTA NGFS EINS KEKA LETD SVSG VSKQ SKKQ KL
Gene ID:	23512
Uniprot:	Q15022
Cellular Location:	Nucleus
Calculated MW:	83kDa
Observed MW:	83kDa

Synonyms:	SUZ12, CHET9, JJAZ1
Background:	This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.

UniProt Protein Function:	SUZ12: a zinc finger protein. A breakpoint translocation of the cognate gene with JAZF1 has been reported in endometrial stromal sarcoma.
UniProt Protein Details:	Protein type:Transcription factor; Oncoprotein; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 17q11.2 Cellular Component: nucleoplasm; ESC/E(Z) complex; sex chromatin; nucleolus; nucleus Molecular Function:histone methyltransferase activity; protein binding; sequence-specific DNA binding; RNA binding; metal ion binding; chromatin binding; methylated histone residue binding Biological Process: histone methylation; negative regulation of cell differentiation; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; positive regulation of cell proliferation; histone ubiquitination; gene expression; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic
NCBI Summary:	This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. [provided by RefSeq, Jul 2009]
UniProt Code:	Q15022
NCBI GenInfo Identifier:	116242808
NCBI Gene ID:	23512
NCBI Accession:	Q15022.3
UniProt Secondary Accession:	Q15022,Q96BD9,
UniProt Related Accession:	Q15022
Molecular Weight:	739
NCBI Full Name:	Polycomb protein SUZ12
NCBI Synonym Full Names:	SUZ12 polycomb repressive complex 2 subunit
NCBI Official Symbol:	SUZ12
NCBI Official Synonym Symbols:	CHET9; JJAZ1
NCBI Protein Information:	polycomb protein SUZ12; chET 9 protein; joined to JAZF1 protein; suppressor of zeste 12 homolog; suppressor of zeste 12 protein homolog; chromatin precipitated E2F target 9 protein
UniProt Protein Name:	Polycomb protein SUZ12
UniProt Synonym Protein Names:	Chromatin precipitated E2F target 9 protein; ChET 9 protein; Joined to JAZF1 protein; Suppressor of zeste 12 protein homolog
Protein Family:	Polycomb protein
UniProt Gene Name:	SUZ12
UniProt Entry Name:	SUZ12_HUMAN

	Western blot analysis of extracts of various cell lines, using SUZ12 Antibody (CAB7786) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and SUZ12 knockout (KO) HeLa cells, using SUZ12 antibody (CAB7786) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using SUZ12 antibody (CAB7786) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using SUZ12 antibody (CAB7786) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using SUZ12 antibody (CAB7786) at dilution of 1:100 (40x lens).