KO Validated Antibodies 1

[KO Validated] SUZ12 Rabbit Polyclonal Antibody (CAB7786)

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SKU:
CAB7786
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
CHET9, IMMAS, JJAZ1, 12
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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[KO Validated] SUZ12 Antibody (CAB7786)

The [KO Validated] SUZ12 Antibody (CAB7786) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting SUZ12 in various biological samples, making it ideal for use in techniques such as Western blotting.SUZ12 is essential for the function of PRC2, which plays a critical role in gene regulation by modifying chromatin structure. Dysregulation of SUZ12 has been implicated in various diseases, including cancer and developmental disorders.

This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] SUZ12 Antibody
SKU:CAB7786
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:CHSR FIFN YVYH PKGA RIDV SINE CYDG SYAG NPQD IHRQ PGFA FSRN GPVK RTPI THIL VCRP KRTK ASMS EFLE SEDG EVEQ QRTY SSGH NRLY FHSD TCLP LRPQ EMEV DSED EKDP EWLR EKTI TQIE EFSD VNEG EKEV MKLW NLHV MKHG FIAD NQMN HACM LFVE NYGQ KIIK KNLC RNFM LHLV SMHD FNLI SIMS IDKA VTKL REMQ QKLE KGES ASPA NEEI TEEQ NGTA NGFS EINS KEKA LETD SVSG VSKQ SKKQ KL
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:100
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CHET9, IMMAS, JJAZ1, 12
Positive Sample:HeLa, BT-474, MCF-7, 293T
Cellular Localization:Nucleus.
Calculated MW:83kDa
Observed MW:83kDa

This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.

Purification MethodAffinity purification
Gene ID23512
RRIDAB_2863561
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] SUZ12 Rabbit pAb (CAB7786) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageWestern blot analysis of lysates from wild type (WT) and SUZ12 knockout (KO) HeLa cells, using [KO Validated] SUZ12 Rabbit pAb (CAB7786) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg SUZ12 antibody (CAB7786). Western blot was performed from the immunoprecipitate using SUZ12 antibody (CAB7786) at a dilution of 1:500.
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