Cell Biology Antibodies 2

SPRR3 Rabbit Polyclonal Antibody (CAB12041)

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SKU:
CAB12041
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
SPRR3
Research Use:
Cell Biology
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Description

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SPRR3 Antibody (CAB12041)

The SPRR3 Antibody (CAB12041) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in immunohistochemistry and Western blot applications. By binding to SPRR3, the antibody enables precise detection and analysis in a variety of cell types, making it an excellent choice for studies in dermatology and skin biology.SPRR3, a member of the small proline-rich protein family, plays a crucial role in maintaining the integrity of the skin barrier and protecting against environmental stresses.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:SPRR3 Antibody
SKU:CAB12041
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSSY QQKQ TFTP PPQL QQQQ VKQP SQPP PQEI FVPT TKEP CHSK VPQP GNTK IPEP GCTK VPEP GCTK VP
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:SPRR3
Positive Sample:Mouse liver, Rat liver
Cellular Localization:Cytoplasm.
Calculated MW:17kDa
Observed MW:26kDa

Predicted to enable structural molecule activity. Predicted to be involved in wound healing. Located in Golgi apparatus and perinuclear region of cytoplasm.

Purification MethodAffinity purification
Gene ID6707
RRIDAB_2758953
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using SPRR3 Rabbit pAb (CAB12041) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunofluorescence analysis of L929 cells using SPRR3 Rabbit pAb (CAB12041) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U2OS cells using SPRR3 Rabbit pAb (CAB12041) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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