Cell Biology Antibodies 10

SPN Rabbit Polyclonal Antibody (CAB6412)

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SKU:
CAB6412
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
LSN, CD43, GALGP, GPL115, LEU-22, SPN
Research Use:
Cell Biology
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Description

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SPN Antibody (CAB6412)

The SPN Antibody (CAB6412) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, is highly specific to human leukosialin and is validated for use in a variety of research applications, including Western blotting.Leukosialin plays a crucial role in regulating immune cell interactions, including T cell activation and trafficking. Its expression has been linked to various immune-related diseases, making it an attractive target for investigation in immunology and cancer research.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:SPN Antibody
SKU:CAB6412
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:LWRR RQKR RTGA LVLS RGGK RNGV VDAW AGPA QVPE EGAV TVTV GGSG GDKG SGFP DGEG SSRR PTLT TFFG RRKS RQGS LAME ELKS GSGP SLKG EEEP LVAS EDGA VDAP APDE PEGG DGAA P
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:LSN, CD43, GALGP, GPL115, LEU-22, SPN
Positive Sample:U-937
Cellular Localization:Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:40kDa
Observed MW:115-130kDa

This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome.

Purification MethodAffinity purification
Gene ID6693
RRIDAB_2767014
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from U-937 cells using SPN Rabbit pAb (CAB6412) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human tonsil using SPN Rabbit pAb (CAB6412) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse spleen using SPN Rabbit pAb (CAB6412) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of Jurkat cells using SPN Rabbit pAb (CAB6412) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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