Cell Biology Antibodies 10

SPINT1 Rabbit Polyclonal Antibody (CAB6411)

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SKU:
CAB6411
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
HAI, HAI1, MANSC2, SPINT1
Research Use:
Cell Biology
Frequently bought together:

Description

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SPINT1 Antibody (CAB6411)

The SPINT1 Antibody (CAB6411) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reacts strongly with human samples, making it an ideal choice for Western blot applications.SPINT1, also known as HAI-1, is a potent inhibitor of certain proteases involved in cell growth and differentiation. Dysregulation of SPINT1 has been linked to various diseases, including cancer and inflammatory conditions.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:SPINT1 Antibody
SKU:CAB6411
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ANVT VTVL STKQ TEDY CLAS NKVG RCRG SFPR WYYD PTEQ ICKS FVYG GCLG NKNN YLRE EECI LACR GVQG PSME RRHP VCSG TCQP TQFR CSNG CCID SFLE CDDT PNCP DASD EAAC EKYT SGFD ELQR IHFP SDKG HCVD LPDT GLCK ESIP RWYY NPFS EHCA RFTY GGCY GNKN NFEE EQQC LESC RGIS KKDV FGLR REIP IPST GSVE MAVA VFLV ICIV VVVA ILGY CFFK NQRK DFHG HHHH PPPT PASS TVST TEDT EHLV YNHT TRPL
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HAI, HAI1, MANSC2, SPINT1
Positive Sample:Mouse liver, Rat brain
Cellular Localization:Secreted.
Calculated MW:58kDa
Observed MW:70kDa

The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms.

Purification MethodAffinity purification
Gene ID6692
RRIDAB_2767013
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using SPINT1 Rabbit pAb (CAB6411) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
Product ImageImmunofluorescence analysis of HeLa cells using SPINT1 Rabbit pAb (CAB6411). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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