Epigenetics & Nuclear Signaling Antibodies 2

SLC25A5 Rabbit Polyclonal Antibody (CAB15639)

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SKU:
CAB15639
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat, Monkey
Synonyms:
T2, T3, 2F1, AAC2, ANT2, SLC25A5
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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SLC25A5 Antibody (CAB15639)

The SLC25A5 Antibody (CAB15639) is a high-quality antibody developed for reliable detection and analysis of target proteins. This cell membrane protein plays a key role in cellular energy metabolism by facilitating the exchange of ADP and ATP across the mitochondrial inner membrane.Raised in rabbits, this antibody exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the SLC25A5 protein, researchers can detect and analyze its expression in various cell types, making it a versatile tool for studies in cellular metabolism, bioenergetics, and mitochondrial function.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Monkey samples.

Product Name:SLC25A5 Antibody
SKU:CAB15639
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat, Monkey
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:EREF RGLG DCLV KIYK SDGI KGLY QGFN VSVQ GIII YRAA YFGI YDTA KGML PDPK NTHI VISW MIAQ TVTA VAGL TSYP FDTV RRRM MMQS GRKG TDIM
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:5000
IF/ICC1:50 - 1:200
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:T2, T3, 2F1, AAC2, ANT2, SLC25A5
Positive Sample:PC-3, U-937, COS-7, Neuro-2a, Rat kidney
Cellular Localization:Mitochondrion Inner Membrane, Multi-Pass Membrane Protein.
Calculated MW:33kDa
Observed MW:34kDa

This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into the cytoplasm. The protein forms a homodimer embedded in the inner mitochondria membrane. Suppressed expression of this gene has been shown to induce apoptosis and inhibit tumor growth. The human genome contains several non-transcribed pseudogenes of this gene.

Purification MethodAffinity purification
Gene ID292
RRIDAB_2763045
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using ANT2/SLC25A5 Rabbit pAb (CAB15639) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Product ImageWestern blot analysis of various lysates, using ANT2/SLC25A5 Rabbit pAb (CAB15639) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse heart using ANT2/SLC25A5 Rabbit pAb (CAB15639) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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