Cell Death Antibodies 1

SGK1 Rabbit Polyclonal Antibody (CAB1025)

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SKU:
CAB1025
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Mouse, Rat
Synonyms:
SGK, SGK1
Research Use:
Cell Death
Frequently bought together:

Description

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SGK1 Antibody (CAB1025)

The SGK1 Antibody (CAB1025) is a high-quality antibody developed for reliable detection and analysis of target proteins. SGK1 is a serine/threonine protein kinase that plays a key role in cell survival, proliferation, and ion transport. This antibody, raised in rabbits, is highly specific for human samples and is validated for use in Western blot applications.By binding specifically to SGK1, this antibody enables researchers to detect and analyze SGK1 expression in a variety of cell types.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.

Product Name:SGK1 Antibody
SKU:CAB1025
Size:20μL, 100μL
Reactivity:Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:LYFV LDYI NGGE LFYH LQRE RCFL EPRA RFYA AEIA SALG YLHS LNIV YRDL KPEN ILLD SQGH IVLT DFGL CKEN IEHN STTS TFCG TPEY LAPE VLHK QPYD RTVD WWCL GAVL YEML YGLP PFYS RNTA EMYD NILN KPLQ LKPN ITNS ARHL LEGL LQKD RTKR LGAK DDFM EIKS HVFF SLIN WDDL INKK ITPP FNPN VSGP NDLR HFDP EFTE EPVP NSIG KSPD SVLV TASV KEAA EAFL GFSY APPT DSFL
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:100 - 1:500
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:SGK, SGK1
Positive Sample:SK-MEL-28, Mouse brain, Rat lung
Cellular Localization:Cell Membrane, Cytoplasm, Endoplasmic Reticulum Membrane, Mitochondrion, Nucleus.
Calculated MW:49kDa
Observed MW:45-60kDa

This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Purification MethodAffinity purification
Gene ID6446
RRIDAB_2757776
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using SGK1 Rabbit pAb (CAB1025) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse kidney using SGK1 Rabbit pAb (CAB1025) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
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