Epigenetics & Nuclear Signaling Antibodies 1

NR2F2 Rabbit Polyclonal Antibody (CAB10251)

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SKU:
CAB10251
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
ARP1, ARP-1, CHTD4, NF-E3, SRXX5, SVP40, COUPTF2, COUPTFB, TFCOUP2, COUPTFII, NR2F2
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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NR2F2 Antibody (CAB10251)

The NR2F2 Antibody (CAB10251) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to NR2F2, this antibody enables the detection and analysis of the protein in different cell types, making it suitable for investigations in developmental biology, metabolism, and cancer research.NR2F2, also known as COUP-TFII, plays a crucial role in regulating gene expression and signaling pathways that control cell fate and tissue development.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:NR2F2 Antibody
SKU:CAB10251
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MAMV VSTW RDPQ DEVP GSQG SQAS QAPP VPGP PPGA PHTP QTPG QGGP ASTP AQTA AGGQ GGPG GPGS DKQQ QQQH IECV VCGD KSSG KHYG QFTC EGCK
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ARP1, ARP-1, CHTD4, NF-E3, SRXX5, SVP40, COUPTF2, COUPTFB, TFCOUP2, COUPTFII, NR2F2
Positive Sample:MCF7, Molt-4(Negative control), Mouse liver, PC-12, C6
Cellular Localization:Nucleus.
Calculated MW:46kDa
Observed MW:47kDa

This gene encodes a member of the steroid thyroid hormone superfamily of nuclear receptors. The encoded protein is a ligand inducible transcription factor that is involved in the regulation of many different genes. Alternate splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID7026
RRIDAB_2757778
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using NR2F2 Rabbit pAb (CAB10251) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageWestern blot analysis of various lysates, using NR2F2 Rabbit pAb (CAB10251) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse liver using NR2F2 Rabbit pAb (CAB10251) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of A431 cells using NR2F2 Rabbit pAb (CAB10251) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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