Cell Biology Antibodies 13

SEMA4A Rabbit Polyclonal Antibody (CAB17205)

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SKU:
CAB17205
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
RP35, SEMB, SEMAB, CORD10, SEMA4A
Research Use:
Cell Biology
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Description

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SEMA4A Antibody (CAB17205)

The SEMA4A Antibody (CAB17205) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By targeting the SEMA4A protein, this antibody enables the detection and analysis of SEMA4A expression in a variety of cell types, making it an essential tool for studies in immunology, neurobiology, and cancer research.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:SEMA4A Antibody
SKU:CAB17205
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:IYAV FTSQ WQVG GTRS SAVC AFSL LDIE RVFK GKYK ELNK ETSR WTTY RGPE TNPR PGSC SVGP SSDK ALTF MKDH FLMD EQVV GTPL LVKS GVEY TRLA VETA QGLD GHSH LVMY LGTT TGSL HKAV VSGD SSAH LVEE IQLF PDPE PVRN LQLA PTQG AVFV GFSG GVWR VPRA NCSV YESC VDCV LARD PHCA WDPE SRTC CLLS APNL NSWK QDME RGNP EWAC ASGP MSRS LRPQ SRPQ IIKE VLAV PNSI LELP C
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RP35, SEMB, SEMAB, CORD10, SEMA4A
Positive Sample:HT-29
Cellular Localization:Extracellular Space, Plasma Membrane.
Calculated MW:84kDa
Observed MW:83kDa

This gene encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Purification MethodAffinity purification
Gene ID64218
RRIDAB_2772175
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HT-29 cells, using SEMA4A Rabbit pAb (CAB17205) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunofluorescence analysis of C6 cells using SEMA4A Rabbit pAb (CAB17205) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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