Immunology Antibodies 2

SATB1 Rabbit Polyclonal Antibody (CAB5800)

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SKU:
CAB5800
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Reactivity:
Human, Mouse, Rat
Synonyms:
DEFDA, KTZSL, SATB1
Research Use:
Immunology
Frequently bought together:

Description

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SATB1 Antibody (CAB5800)

The SATB1 Antibody (CAB5800) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blotting and immunohistochemistry applications.SATB1, or Special AT-rich Sequence-Binding Protein 1, plays a vital role in the organization of chromatin structure and the regulation of gene expression. Its involvement in various cellular processes, including cell differentiation, proliferation, and immune response, makes it a valuable target for research in immunology, cancer, and developmental biology.

This antibody is validated for use in WB, IHC-P, IP, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:SATB1 Antibody
SKU:CAB5800
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:NAVH HHGD RPPH IIHV PAEQ IQQQ QQQQ QQQQ QQQQ APPP PQPQ QQPQ TGPR LPPR QPTV ASPA ESDE ENRQ KTRP RTKI SVEA LGIL QSFI QDVG LYPD EEAI QTLS AQLD LPKY TIIK FFQN QRYY LKHH GKLK DNSG LEVD VAEY KEEE LLKD LEES VQDK NTNT LFSV KLEE ELSV EGNT DINT DLKD
Tested Applications:WB IHC-P IP ELISA IF-P
Recommended Dilution:
WB1:500 - 1:5000
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
IF-P1:50 - 1:200
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:DEFDA, KTZSL, SATB1
Positive Sample:Jurkat
Cellular Localization:Nucleus, Nucleus Matrix, Pml Body.
Calculated MW:86kDa
Observed MW:100kDa

This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression.

Purification MethodAffinity purification
Gene ID6304
RRIDAB_2766552
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Jurkat cells, using SATB1 Rabbit pAb (CAB5800) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat brain using SATB1 Rabbit pAb (CAB5800) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of paraffin-embedded rat thymus using SATB1 Rabbit pAb (CAB5800) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg SATB1 antibody (CAB5800). Western blot was performed from the immunoprecipitate using SATB1 antibody (CAB5800) at a dilution of 1:500.
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