Epigenetics & Nuclear Signaling Antibodies 4

SART1 Rabbit Polyclonal Antibody (CAB8569)

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SKU:
CAB8569
Product Type:
Antibody
Applications:
WB
Applications:
IP
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
HAF, Ara1, HOMS1, Snu66, SART1259, SNRNP110, SART1
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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SART1 Antibody (CAB8569)

The SART1 Antibody (CAB8569) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By targeting the SART1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in molecular biology and cancer research.SART1, also known as squamous cell carcinoma antigen recognized by T cells, plays a crucial role in the regulation of gene expression and the maintenance of genomic stability.

This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:SART1 Antibody
SKU:CAB8569
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MGSS KKHR GEKE AAGT TAAA GTGG ATEQ PPRH REHK KHKH RSGG SGGS GGER RKRS RERG GERG SGRR GAEA EARS STHG RERS QAEP SERR VKRE KRDD GYEA AASS KTSS GDAS SLSI EETN KLRA KLGL KPLE VNAI KKEA GTKE EPVT ADVI NPMA LRQR EELR EKLA AAKE KRLL NQKL GKIK TLGE DDPW LDDT AAWI ERSR QLQK EKDL AEKR AKLL EEMD QEFG VSTL VEEE FGQR RQDL YSAR DLQ
Tested Applications:WB IHC-P IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HAF, Ara1, HOMS1, Snu66, SART1259, SNRNP110, SART1
Positive Sample:HeLa, Jurkat, HepG2, Mouse liver, Mouse brain, Mouse testis, Rat brain
Cellular Localization:Nucleus.
Calculated MW:90kDa
Observed MW:120kDa

This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway.

Purification MethodAffinity purification
Gene ID9092
RRIDAB_2772144
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using SART1 Rabbit pAb (CAB8569) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human esophageal using SART1 Rabbit pAb (CAB8569) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat brain using SART1 Rabbit pAb (CAB8569) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunoprecipitation analysis of 150 μg extracts of Jurkat cells using 3 μg SART1 antibody (CAB8569). Western blot was performed from the immunoprecipitate using SART1 antibody (CAB8569) at a dilution of 1:1000.
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