Cell Biology Antibodies 13

RPL35 Rabbit Polyclonal Antibody (CAB17632)

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SKU:
CAB17632
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
L35, uL29, DBA19, RPL35
Research Use:
Cell Biology
Frequently bought together:

Description

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RPL35 Antibody (CAB17632)

The RPL35 Antibody (CAB17632) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to the RPL35 protein, this antibody allows for the detection and analysis of RPL35 in a variety of cell types, making it an excellent choice for studies in molecular biology and cancer research.RPL35 plays a crucial role in the ribosomal machinery, contributing to the translation of mRNA into protein.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:RPL35 Antibody
SKU:CAB17632
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:VRKS IARV LTVI NQTQ KENL RKFY KGKK YKPL DLRP KKTR AMRR RLNK HEEN LKTK KQQR KERL YPLR KYA
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:L35, uL29, DBA19, RPL35
Positive Sample:HeLa, Jurkat, A-549
Cellular Localization:Cytoplasm, Cytosol, Cytosolic Ribosome, Nucleolus.
Calculated MW:15kDa
Observed MW:16kDa

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Purification MethodAffinity purification
Gene ID11224
RRIDAB_2772082
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using RPL35 Rabbit pAb (CAB17632) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RPL35 Rabbit pAb (CAB17632) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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