Immunology Antibodies 2

RNGTT Rabbit Polyclonal Antibody (CAB6431)

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SKU:
CAB6431
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Reactivity:
Human, Mouse, Rat
Synonyms:
HCE, HCE1, hCAP, CAP1A, RNGTT
Research Use:
Immunology
Frequently bought together:

Description

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RNGTT Antibody (CAB6431)

The RNGTT Antibody (CAB6431) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the mRNA capping enzyme, this antibody enables the detection and analysis of mRNA capping enzyme levels in various cell types.The mRNA capping enzyme is essential for the proper function of mRNA molecules, playing a key role in gene expression regulation.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:RNGTT Antibody
SKU:CAB6431
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAHN KIPP RWLN CPRR GQPV AGRF LPLK TMLG PRYD SQVA EENR FHPS MLSN YLKS LKVK MGLL VDLT NTSR FYDR NDIE KEGI KYIK LQCK GHGE CPTT ENTE TFIR LCER FNER NPPE LIGV HCTH GFNR TGFL ICAF LVEK MDWS IEAA VATF AQAR PPGI YKGD YLKE LFRR YGDI EEAP PPPL LPDW CFED DEDE DEDE DGKK ESEP GSSA SFGK RRKE RLKL GAIF LEGV TVKG VTQV TTQP KLGE VQQK CHQF
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HCE, HCE1, hCAP, CAP1A, RNGTT
Positive Sample:MCF7, THP-1, 293T, Jurkat, HeLa, Mouse brain, Mouse spleen, Mouse kidney, Rat brain
Cellular Localization:Nucleus.
Calculated MW:69kDa
Observed MW:69kDa

Enables mRNA guanylyltransferase activity and triphosphatase activity. Involved in 7-methylguanosine mRNA capping. Predicted to be located in nucleoplasm.

Purification MethodAffinity purification
Gene ID8732
RRIDAB_2767033
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using RNGTT Rabbit pAb (CAB6431) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunofluorescence analysis of MCF7 cells using RNGTT Rabbit pAb (CAB6431). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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