Signal Transduction Antibodies 3

RFK Rabbit Polyclonal Antibody (CAB9141)

(No reviews yet) Write a Review
SKU:
CAB9141
Product Type:
Antibody
Applications:
WB
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse
Synonyms:
RIFK, RFK
Research Use:
Signal Transduction
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

RFK Antibody (CAB9141)

The RFK Antibody (CAB9141) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the RFK protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an excellent choice for studies in biochemistry and molecular biology.RFK plays a crucial role in the synthesis of tetrahydrofolate, a key molecule in one-carbon metabolism essential for DNA synthesis and cell growth.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:RFK Antibody
SKU:CAB9141
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MRHL PYFC RGQV VRGF GRGS KQLG IPTA NFPE QVVD NLPA DIST GIYY GWAS VGSG DVHK MVVS IGWN PYYK NTKK SMET HIMH TFKE DFYG EILN VAIV GYLR PEKN FDSL ESLI SAIQ GDIE EAKK RLEL PEHL KIKE DNFF QVSK SKIM NGH
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RIFK, RFK
Positive Sample:U-87MG, HeLa, 293T, Mouse small intestine, Mouse brain
Cellular Localization:Cytoplasm.
Calculated MW:18kDa
Observed MW:14kDa

Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).

Purification MethodAffinity purification
Gene ID55312
RRIDAB_2771988
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using RFK Rabbit pAb (CAB9141) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Liu, Y., et al.Targeting Riboflavin Kinase Induces Ferroptosis and Apoptosis in Triple-Negative Breast CancerCancer Metabolism and Cell Death Mechanisms 2025View Citation
View AllClose

0 Reviews

View AllClose

Related Products