Epigenetics & Nuclear Signaling Antibodies 3

RFC4 Rabbit Polyclonal Antibody (CAB5485)

(No reviews yet) Write a Review
SKU:
CAB5485
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Rat
Synonyms:
A1, RFC37, RFC4
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

RFC4 Antibody (CAB5485)

The RFC4 Antibody (CAB5485) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. It specifically targets the RFC4 protein, allowing for accurate detection and analysis in a variety of cell types.RFC4 plays a crucial role in maintaining genomic stability by assisting in the replication of DNA and ensuring proper repair of damaged DNA.

This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Rat samples.

Product Name:RFC4 Antibody
SKU:CAB5485
Size:20μL, 100μL
Reactivity:Human, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:DKIQ QQRL LDIA KKEN VKIS DEGI AYLV KVSE GDLR KAIT FLQS ATRL TGGK EITE KVIT DIAG VIPA EKID GVFA ACQS GSFD KLEA VVKD LIDE GHAA TQLV NQLH DVVV ENNL SDKQ KSII TEKL AEVD KCLA DGAD EHLQ LISL CATV MQQL SQNC
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:100
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:A1, RFC37, RFC4
Positive Sample:HeLa, SW480, 22Rv1, SW620, K-562, HepG2, MCF7
Cellular Localization:Nucleus.
Calculated MW:40kDa
Observed MW:40kDa

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.

Purification MethodAffinity purification
Gene ID5984
RRIDAB_2766285
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using RFC4 Rabbit pAb (CAB5485) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunofluorescence analysis of U2OS cells using RFC4 Rabbit pAb (CAB5485). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunoprecipitation analysis of 200 μg extracts of K562 cells using 1 μg RFC4 antibody (CAB5485). Western blot was performed from the immunoprecipitate using RFC4 antibody (CAB5485) at a dilution of 1:1000.
View AllClose

0 Reviews

View AllClose

Related Products