The RanGAP1 Monoclonal Antibody (CAB8758) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, generated in rabbits, is highly specific and validated for use in immunofluorescence and immunohistochemistry applications. It binds specifically to the RANGAP1 protein, allowing for precise detection and analysis in various cell types.RANGAP1 plays a crucial role in the process of cell division, specifically in the regulation of the breakdown of the nuclear membrane during mitosis.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RanGAP1 Monoclonal Antibody
SKU:
CAB8758
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1292
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
5905
RRID
AB_2863603
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using RanGAP1 Rabbit mAb (CAB8758) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using RanGAP1 Rabbit mAb (CAB8758) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using RanGAP1 Rabbit mAb (CAB8758) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using RanGAP1 Rabbit mAb (CAB8758) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of C6 cells using RanGAP1 Rabbit mAb (CAB8758) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using RanGAP1 Rabbit mAb (CAB8758) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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