Cell Biology Antibodies 3

RanGAP1 Rabbit Polyclonal Antibody (CAB13347)

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SKU:
CAB13347
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Synonyms:
SD, Fug1, RANGAP, RanGAP1
Research Use:
Cell Biology
Frequently bought together:

Description

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RanGAP1 Antibody (CAB13347)

The RanGAP1 Antibody (CAB13347) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity and sensitivity for detecting RANGAP1 in human samples, making it ideal for use in Western blot applications.RANGAP1 is essential for the proper localization of the RanGTPase on the nuclear envelope during mitosis, facilitating the regulation of nuclear transport and ensuring the fidelity of cell division.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:RanGAP1 Antibody
SKU:CAB13347
Size:20μL, 100μL
Reactivity:Human
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:PQQR GQGE KSAT PSRK ILDP NTGE PAPV LSSP PPAD VSTF LAFP SPEK LLRL GPKS SVLI AQQT DTSD PEKV VSAF LKVS SVFK DEAT VRMA VQDA VDAL MQKA FNSS SFNS NTFL TRLL VHMG LLKS EDKV KAIA NLYG PLMA LNHM VQQD YFPK ALAP LLLA FVTK PNSA LESC SFAR HSLL QTLY KV
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:SD, Fug1, RANGAP, RanGAP1
Positive Sample:BT-474, HeLa, MCF7, SH-SY5Y, A-549, Jurkat
Cellular Localization:Chromosome, Cytoplasm, Cytoplasmic Side, Nucleus Membrane, Peripheral Membrane Protein, Centromere, Cytoskeleton, Kinetochore, Spindle Pole.
Calculated MW:64kDa
Observed MW:64/90kDa

This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID5905
RRIDAB_2760204
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using RanGAP1 Rabbit pAb (CAB13347) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human esophageal cancer using RanGAP1 Rabbit pAb (CAB13347) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of MCF-7 cells using RanGAP1 Rabbit pAb (CAB13347).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution.
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