Cell Biology Antibodies 14

RALGDS Rabbit Polyclonal Antibody (CAB17963)

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SKU:
CAB17963
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
RGF, RGDS, RalGEF, RALGDS
Research Use:
Cell Biology
Frequently bought together:

Description

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RALGDS Antibody (CAB17963)

The RALGDS Antibody (CAB17963) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for RalGDS and is suitable for use in various applications, including Western blotting and immunohistochemistry.RalGDS is a guanine nucleotide exchange factor that activates the small GTPase Ral, leading to downstream signaling events that regulate cell proliferation, migration, and survival.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:RALGDS Antibody
SKU:CAB17963
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:CNSS SALP LYNQ QVGD CCII RVSL DVDN GNMY KSIL VTSQ DKAP AVIR KAMD KHNL EEEE PEDY ELLQ ILSD DRKL KIPE NANV FYAM NSTA NYDF VLKK RTFT KGVK VKHG ASST LPRM KQKG LKIA KGIF
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RGF, RGDS, RalGEF, RALGDS
Positive Sample:U-87MG, Mouse ovary, Mouse lung
Cellular Localization:Cytosol, Nucleus, Plasma Membrane.
Calculated MW:101kDa
Observed MW:110kDa

Guanine nucleotide dissociation stimulators (GDSs, or exchange factors), such as RALGDS, are effectors of Ras-related GTPases (see MIM 190020) that participate in signaling for a variety of cellular processes.

Purification MethodAffinity purification
Gene ID5900
RRIDAB_2861765
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using RALGDS Rabbit pAb (CAB17963) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse testis using RALGDS Rabbit pAb (CAB17963) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human thyroid cancer using RALGDS Rabbit pAb (CAB17963) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of L929 cells using RALGDS Rabbit pAb (CAB17963) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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