Cell Biology Antibodies 14

RAB11FIP5 Rabbit Polyclonal Antibody (CAB18142)

(No reviews yet) Write a Review
SKU:
CAB18142
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
GAF1, pp75, RIP11, gaf-1, rab11-FIP5, RAB11FIP5
Research Use:
Cell Biology
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

RAB11FIP5 Antibody (CAB18142)

The RAB11FIP5 Antibody (CAB18142) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific for human samples and has been validated for use in various applications, including Western blot and immunofluorescence.Rab11FIP5, also known as Rab11 family-interacting protein 5, plays a crucial role in the recycling of endocytic vesicles and the regulation of membrane trafficking pathways. Its involvement in these processes makes it a key player in cell physiology and is implicated in various cellular processes, including cell migration, cytokinesis, and cell adhesion.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:RAB11FIP5 Antibody
SKU:CAB18142
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:HRSS ISGS LPSS GSLQ AVSS RFSE EGPR STDD TWPR GSRS NSSS EAVL GQEE LSAQ AKVL APGA SHPG EEEG ARLP EGKP VQVA TPIV ASSE AVAE KEGA RKEE RKPR MGLF HHHH QGLS RSEL GRRS SLGE KGGP ILGA SPHH SSSG EEKA KSSW FGLR EAKD PTQK PSPH PVKP LSAA PVEG SPDR KQSR SSLS IALS SGLE KLKT VTSG SIQP VTQA PQAG QMVD TKRL KDSA VLDQ SAKY YHLT HDEL ISLL LQRE RELS QRDE HVQE LESY IDRL LVRI METS PTLL QIPP GPPK
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:GAF1, pp75, RIP11, gaf-1, rab11-FIP5, RAB11FIP5
Positive Sample:A-431, 293F, Mouse kidney, Mouse lung, Rat testis
Cellular Localization:Cytoplasm, Cytoplasmic Vesicle, Early Endosome Membrane, Golgi Apparatus Membrane, Mitochondrion Membrane, Peripheral Membrane Protein, Recycling Endosome Membrane, Secretory Vesicle Membrane.
Calculated MW:70kDa
Observed MW:70-90kDa

Enables gamma-tubulin binding activity. Involved in cellular response to acidic pH; negative regulation of adiponectin secretion; and regulation of protein localization to cell surface. Located in centriolar satellite and mitochondrial outer membrane.

Purification MethodAffinity purification
Gene ID26056
RRIDAB_2861933
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using RAB11FIP5 Rabbit pAb (CAB18142) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageWestern blot analysis of various lysates, using RAB11FIP5 Rabbit pAb (CAB18142) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using RAB11FIP5 Rabbit pAb (CAB18142) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products