Cell Biology Antibodies 18

PTRF Rabbit Polyclonal Antibody (CAB18331)

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SKU:
CAB18331
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
CGL4, PTRF, CAVIN, FKSG13, cavin-1
Research Use:
Cell Biology
Frequently bought together:

Description

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PTRF Antibody (CAB18331)

The PTRF Antibody (CAB18331) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to PTRF, this antibody enables the detection and analysis of the protein in various cell types, making it an invaluable asset for studies in cell biology, lipid metabolism, and signaling pathways.PTRF, also known as Polymerase I and Transcript Release Factor, plays a crucial role in the organization and function of caveolae, lipid-rich domains on the plasma membrane. These structures are involved in diverse cellular processes such as endocytosis, signal transduction, and lipid homeostasis.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:PTRF Antibody
SKU:CAB18331
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ELSS DEAV EVEE VIEE SRAE RIKR SGLR RVDD FKKA FSKE KMEK TKVR TREN LEKT RLKT KENL EKTR HTLE KRMN KLGT R
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CGL4, PTRF, CAVIN, FKSG13, cavin-1
Positive Sample:HT-1080, HeLa, A-549, Mouse stomach, Mouse lung, Mouse heart, Rat heart
Cellular Localization:Cell Membrane, Cytoplasm, Endoplasmic Reticulum, Membrane, Microsome, Mitochondrion, Nucleus, Caveola, Cytosol.
Calculated MW:43kDa
Observed MW:55kDa

This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy.

Purification MethodAffinity purification
Gene ID284119
RRIDAB_2862102
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using PTRF Rabbit pAb (CAB18331) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human placenta using PTRF Rabbit pAb (CAB18331) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells using PTRF Rabbit pAb (CAB18331) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of L929 cells using PTRF Rabbit pAb (CAB18331) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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