Cell Biology Antibodies 5

PTCH1 Rabbit Polyclonal Antibody (CAB14772)

(No reviews yet) Write a Review
SKU:
CAB14772
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Mouse, Rat
Synonyms:
PTC, BCNS, PTC1, PTCH, NBCCS, PTCH1
Research Use:
Cell Biology
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

PTCH1 Antibody (CAB14772)

The PTCH1 Antibody (CAB14772) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for Western blot applications.PTCH1 is a transmembrane receptor protein that serves as a negative regulator of the Hedgehog pathway, controlling the activity of downstream signaling molecules. Dysregulation of PTCH1 has been implicated in various cancers, developmental disorders, and degenerative diseases, highlighting its importance in cellular growth and differentiation processes.

This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.

Product Name:PTCH1 Antibody
SKU:CAB14772
Size:20μL, 100μL
Reactivity:Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:RVRD GLDL TDIV PRET REYD FIAA QFKY FSFY NMYI VTQK ADYP NIQH LLYD LHRS FSNV KYVM LEEN KQLP KMWL HYFR DWLQ GLQD AFDS DWET GKIM PNNY KNGS DDGV LAYK LLVQ TGSR DKPI DISQ LTKQ RLVD ADGI INPS AFYI YLTA WVSN DPVA YAAS QANI RPHR PEWV HDKA DYMP ETRL RIPA AEPI E
Tested Applications:WB ELISA IF-P
Recommended Dilution:
WB1:500 - 1:1000
IF-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PTC, BCNS, PTC1, PTCH, NBCCS, PTCH1
Positive Sample:Rat skeletal muscle
Cellular Localization:Membrane, Multi-Pass Membrane Protein.
Calculated MW:161kDa
Observed MW:160kDa

This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly.

Purification MethodAffinity purification
Gene ID5727
RRIDAB_2761648
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Rat skeletal muscle, using PTCH1 Rabbit pAb (CAB14772) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunofluorescence analysis of paraffin-embedded mouse embryos using PTCH1 Rabbit pAb (CAB14772) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products