Immunology Antibodies 2

PSMA7 Rabbit Polyclonal Antibody (CAB4052)

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SKU:
CAB4052
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
C6, HSPC, RC6-1, XAPC7, HEL-S-276, PSMA7
Research Use:
Immunology
Frequently bought together:

Description

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PSMA7 Antibody (CAB4052)

The PSMA7 Antibody (CAB4052) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the PSMA7 protein, this antibody enables accurate detection and analysis in various cell types, making it an ideal tool for studying proteasome function in the fields of biochemistry and cell biology.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:PSMA7 Antibody
SKU:CAB4052
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSYD RAIT VFSP DGHL FQVE YAQE AVKK GSTA VGVR GRDI VVLG VEKK SVAK LQDE RTVR KICA LDDN VCMA FAGL TADA RIVI NRAR VECQ SHRL TVED PVTV EYIT RYIA SLKQ RYTQ SNGR RPFG ISAL IVGF DFDG TPRL YQTD PSGT YHAW KANA IGRG AKSV REFL EKNY TDEA IETD DLTI KLVI KALL EVVQ SGGK NIEL AVMR RDQS LKIL NPEE IEKY VAEI EKEK EENE KKKQ KKAS
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:100
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:C6, HSPC, RC6-1, XAPC7, HEL-S-276, PSMA7
Positive Sample:U-87MG, 293T, MCF7, Mouse brain, Mouse liver, Mouse lung, Rat liver
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:28kDa
Observed MW:30kDa

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the peptidase T1A family that functions as a 20S core alpha subunit. The encoded protein interacts with the hepatitis B virus X protein and plays a role in regulating hepatitis C virus internal ribosome entry site (IRES) activity, an activity essential for viral replication. The encoded protein also plays a role in the cellular stress response by regulating hypoxia-inducible factor-1alpha. A pseudogene of this gene is located on the long arm of chromosome 9.

Purification MethodAffinity purification
Gene ID5688
RRIDAB_2765472
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using PSMA7 Rabbit pAb (CAB4052) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageWestern blot analysis of lysates from rat liver, using PSMA7 Rabbit pAb (CAB4052) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human liver cancer using PSMA7 Rabbit pAb (CAB4052) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse liver using PSMA7 Rabbit pAb (CAB4052) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using PSMA7 Rabbit pAb (CAB4052) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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