Epigenetics & Nuclear Signaling Antibodies 3

PRPF3 Rabbit Polyclonal Antibody (CAB5482)

(No reviews yet) Write a Review
SKU:
CAB5482
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
PRP3, RP18, HPRP3, Prp3p, HPRP3P, SNRNP90, PRPF3
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

PRPF3 Antibody (CAB5482)

The PRPF3 Antibody (CAB5482) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for Western blot applications. By binding specifically to the PRPF3 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential reagent for studies in molecular biology and genetic research.PRPF3's key role in the splicing machinery makes it a key player in the regulation of gene expression and the maintenance of cellular homeostasis.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:PRPF3 Antibody
SKU:CAB5482
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MALS KREL DELK PWIE KTVK RVLG FSEP TVVT AALN CVGK GMDK KKAA DHLK PFLD DSTL RFVD KLFE AVEE GRSS RHSK SSSD RSRK RELK EVFG DDSE ISKE SSGV KKRR IPRF EEVE EEPE VIPG PPSE SPGM LTKL QIKQ MMEA ATRQ IEER KKQL SFIS PPTP QPKT PSSS QPER LPIG NTIQ PSQA ATFM NDAI EKAR KAAE LQAR IQAQ LALK PGLI GNAN MVGL ANLH AMGI APPK VELK DQTK PTPL ILDE QGRT VDAT GKEI ELTH RMPT
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PRP3, RP18, HPRP3, Prp3p, HPRP3P, SNRNP90, PRPF3
Positive Sample:THP-1
Cellular Localization:Nucleus Speckle.
Calculated MW:78kDa
Observed MW:100kDa

The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18.

Purification MethodAffinity purification
Gene ID9129
RRIDAB_2766282
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from THP-1 cells, using PRPF3 Rabbit pAb (CAB5482) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat brain using PRPF3 Rabbit pAb (CAB5482) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse brain using PRPF3 Rabbit pAb (CAB5482) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of U2OS cells using PRPF3 Rabbit pAb (CAB5482). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products