Epigenetics & Nuclear Signaling Antibodies 4

POLG2 Rabbit Polyclonal Antibody (CAB6695)

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SKU:
CAB6695
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
HP55, POLB, PEOA4, POLGB, MTPOLB, MTDPS16, MTDPS16A, MTDPS16B, POLG-BETA, POLG2
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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POLG2 Antibody (CAB6695)

The POLG2 Antibody (CAB6695) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications. By facilitating the detection and analysis of POLG2 protein expression in various cell types, this antibody is essential for advancing research in the fields of genetics, mitochondrial biology, and neurodegenerative diseases.POLG2 is an essential component of the DNA polymerase gamma complex, which is responsible for maintaining the integrity of mitochondrial DNA.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:POLG2 Antibody
SKU:CAB6695
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:HPVF DTKQ IRNG VKSI GEKT EASL VWFT PPRT SNQW LDFW LRHR LQWW RKFA MSPS NFSS SDCQ DEEG RKGN KLYY NFPW GKEL IETL WNLG DHEL LHMY PGNV SKLH GRDG RKNV VPCV LSVN GDLD RGML AYLY DSFQ LTEN SFTR KKNL HRKV LKLH PCLA PIKV ALDV GRGP TLEL RQVC QGLF NELL ENGI SVWP GYLE TMQS SLEQ LYSK YDEM SILF TVLV TETT LENG LIHL RSRD TTMK EMMH ISKL KDFL IKYI SSAK NV
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:10 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HP55, POLB, PEOA4, POLGB, MTPOLB, MTDPS16, MTDPS16A, MTDPS16B, POLG-BETA, POLG2
Positive Sample:293T, MCF7
Cellular Localization:Mitochondrion.
Calculated MW:55kDa
Observed MW:55kDa

This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions.

Purification MethodAffinity purification
Gene ID11232
RRIDAB_2767279
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using POLG2 Rabbit pAb (CAB6695) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat heart using POLG2 Rabbit pAb (CAB6695) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse testis using POLG2 Rabbit pAb (CAB6695) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse kidney using POLG2 Rabbit pAb (CAB6695) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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