Cell Biology Antibodies 17

PNPO Rabbit Polyclonal Antibody (CAB18495)

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SKU:
CAB18495
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
PDXPO, HEL-S-302, PNPO
Research Use:
Cell Biology
Frequently bought together:

Description

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PNPO Antibody (CAB18495)

The PNPO Antibody (CAB18495) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed in rabbits, offers high reactivity with human samples and is validated for use in Western blotting applications. By specifically binding to the PNPO protein, this antibody enables accurate detection and analysis in various cell types, making it an invaluable asset for studies in biochemistry and metabolism research.PNPO plays a crucial role in the conversion of pyridoxine to pyridoxal 5'-phosphate, the biologically active form of vitamin B6. Dysregulation of PNPO activity has been linked to various neurological disorders, making it a promising target for therapeutic interventions.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:PNPO Antibody
SKU:CAB18495
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MTCW LRGV TATF GRPA EWPG YLSH LCGR SAAM DLGP MRKS YRGD REAF EETH LTSL DPVK QFAA WFEE AVQC PDIG EANA MCLA TCTR DGKP SARM LLLK GFGK DGFR FFTN FESR KGKE LDSN PFAS LVFY WEPL NRQV RVEG PVKK LPEE EAEC YFHS RPKS SQIG AVVS HQSS VIPD REYL RKKN EELE QLYQ DQEV PKPK SWGG YVLY PQVM EFWQ GQTN RLHD RIVF RRGL PTGD SPLG PMTH RGEE DWLY ERLA P
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PDXPO, HEL-S-302, PNPO
Positive Sample:HepG2, 293T, Mouse brain, Mouse kidney, Mouse liver, Rat brain, Rat kidney, Rat liver
Cellular Localization:Cytosol.
Calculated MW:30kDa
Observed MW:30kDa

The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy.

Purification MethodAffinity purification
Gene ID55163
RRIDAB_2862261
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using PNPO Rabbit pAb (CAB18495) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunofluorescence analysis of L929 cells using PNPO Rabbit pAb (CAB18495) at dilution of 1:100. Blue: DAPI for nuclear staining.
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