Cell Biology Antibodies 10

PLIN2 Rabbit Polyclonal Antibody (CAB6276)

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SKU:
CAB6276
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
ADFP, ADRP, Perilipin-2
Research Use:
Cell Biology
Frequently bought together:

Description

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PLIN2 Antibody (CAB6276)

The PLIN2 Antibody (CAB6276) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to perilipin-2, researchers can effectively detect and analyze this important protein in a variety of cell types.Perilipin-2, also known as adipophilin or ADRP, is involved in regulating lipid droplet formation and breakdown, making it essential for understanding processes such as lipolysis and fat storage.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:PLIN2 Antibody
SKU:CAB6276
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MMQL VSSG VENA LTKS ELLV EQYL PLTE EELE KEAK KVEG FDLV QKPS YYVR LGSL STKL HSRA YQQA LSRV KEAK QKSQ QTIS QLHS TVHL IEFA RKNV YSAN QKIQ DAQD KLYL SWVE WKRS IGYD DTDE SHCA EHIE SRTL AIAR NLTQ QLQT TCHT LLSN IQGV PQNI QDQA KHMG VMAG DIYS VFRN AASF KEVS DSLL TSSK GQLQ KMKE SLDD VMDY LVNN TPLN WLVG PFYP QLTE SQNA QDQG AEMD KSSQ ETQR SEHK TH
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:100 - 1:500
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ADFP, ADRP, Perilipin-2
Positive Sample:HepG2, Rat heart
Cellular Localization:Membrane, Peripheral Membrane Protein.
Calculated MW:48kDa
Observed MW:48kDa

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.

Purification MethodAffinity purification
Gene ID123
RRIDAB_2766881
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Rat heart, using Perilipin-2 Rabbit pAb (CAB6276) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageWestern blot analysis of lysates from HepG2 cells, using Perilipin-2 Rabbit pAb (CAB6276) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunofluorescence analysis of C6 cells using Perilipin-2 Rabbit pAb (CAB6276) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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