Cell Death Antibodies 1

PIM1 Rabbit Polyclonal Antibody (CAB0175)

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SKU:
CAB0175
Product Type:
Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Synonyms:
PIM, PIM1
Research Use:
Cell Death
Frequently bought together:

Description

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PIM1 Antibody (CAB0175)

The PIM1 Antibody (CAB0175) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reacts strongly with human samples, making it an ideal choice for Western blot applications. By binding to the Pim1 protein, this antibody allows for precise detection and analysis in various cell types, facilitating in-depth research in the fields of oncology and cell signaling.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.

Product Name:PIM1 Antibody
SKU:CAB0175
Size:20μL, 100μL
Reactivity:Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MLLS KINS LAHL RAAP CNDL HATK LAPG KEKE PLES QYQV GPLL GSGG FGSV YSGI RVSD NLPV AIKH VEKD RISD WGEL PNGT RVPM EVVL LKKV SSGF SGVI RLLD WFER PDSF VLIL ERPE PVQD LFDF ITER GALQ EELA RSFF WQVL EAVR HCHN CGVL HRDI KDEN ILID LNRG ELKL IDFG SGAL LKDT VYTD FDGT RVYS PPEW IRYH RYHG RSAA VWSL GILL YDMV CGDI PFEH DEEI IRGQ VFFR QRVS SECQ HLIR WCLA LRPS DRPT FEEI QNHP WMQD VLLP QETA EIHL HSLS PGPS K
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PIM, PIM1
Positive Sample:Rat kidney
Cellular Localization:Cell Membrane, Cytoplasm, Nucleus.
Calculated MW:36kDa
Observed MW:38kDa

The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).

Purification MethodAffinity purification
Gene ID5292
RRIDAB_2757001
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from rat kidney, using PIM1 Rabbit pAb (CAB0175) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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