Developmental Biology

ODF2 Rabbit Polyclonal Antibody (CAB15630)

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SKU:
CAB15630
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
CT134, ODF84, ODF2/1, ODF2/2, ODF2
Research Use:
Developmental Biology
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Description

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ODF2 Antibody (CAB15630)

The ODF2 Antibody (CAB15630) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards ODF2 in human samples, making it suitable for Western blot applications. By binding to the ODF2 protein, this antibody enables the detection and analysis of ODF2 in various cell types, allowing for in-depth studies in cell biology and development research.ODF2, also known as outer dense fiber 2, plays a crucial role in centriole organization and function, contributing to the accurate segregation of chromosomes during cell division.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ODF2 Antibody
SKU:CAB15630
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:IISD LRSR IEHQ GDKL EMAR EKHQ ASQK ENKQ LSLK VDEL ERKL EATS AQNI EFLQ VIAK REEA IHQS QLRL EEKT RECG TLAR QLES AIED ARRQ VEQT KEHA LSKE RAAQ NKIL DLET QLSR TKTE LSQL RRSR DDAD RRYQ SRLQ DLKD RLEQ SEST NRSM QNYV QFLK SSYA NVFG DGPY STFL TSSP IRSR SPPA
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CT134, ODF84, ODF2/1, ODF2/2, ODF2
Positive Sample:HepG2, Jurkat, 293T, Mouse brain, Mouse lung, Mouse testis, Rat testis
Cellular Localization:Centriolar Subdistal Appendage, Centriole, Centrosome, Ciliary Basal Body, Ciliary Transition Fiber, Cilium, Cytosol, Nucleus, Sperm Flagellum, Spindle Pole.
Calculated MW:95kDa
Observed MW:80kDa

The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants. The longer transcripts, also known as 'Cenexins', encode proteins with a C-terminal extension that are differentially targeted to somatic centrioles and thought to be crucial for the formation of microtubule organizing centers.

Purification MethodAffinity purification
Gene ID4957
RRIDAB_2763038
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using ODF2 Rabbit pAb (CAB15630) at dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Product ImageImmunofluorescence analysis of HeLa cells using ODF2 Rabbit pAb (CAB15630) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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