Developmental Biology

NOTCH4 Rabbit Polyclonal Antibody (CAB8303)

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SKU:
CAB8303
Product Type:
Antibody
Applications:
WB
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Developmental Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
INT3, NOTCH4
Research Use:
Developmental Biology
Frequently bought together:

Description

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NOTCH4 Antibody (CAB8303)

The NOTCH4 Antibody (CAB8303) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the Notch4 protein, this antibody enables accurate detection and analysis in various cell types, making it an essential component for investigations in the fields of cell biology, development, and cancer research.Notch4 is a transmembrane receptor involved in critical cellular functions such as cell differentiation, proliferation, and apoptosis.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:NOTCH4 Antibody
SKU:CAB8303
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:PPEA RHKA TPGR EAGP FPRA RTVS VSVP PHGG GALP RCRT LSAG AGPR GGGA CLQA RTWS VDLA ARGG GAYS HCRS LSGV GAGG GPTP RGRR FSAG MRGP RPNP AIMR GRYG VAAG RGGR VSTD DWPC DWVA LGAC GSAS NIPI PPPC LTPS PERG SPQL DCGP PALQ EMPI NQGG EGKK
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:100 - 1:500
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:INT3, NOTCH4
Positive Sample:Mouse lung
Cellular Localization:Cell Membrane, Nucleus, Single-Pass Type I Membrane Protein.
Calculated MW:210kDa
Observed MW:80kDa

This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Purification MethodAffinity purification
Gene ID4855
RRIDAB_2770622
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Mouse lung, using NOTCH4 Rabbit pAb (CAB8303) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageImmunofluorescence analysis of L929 cells using NOTCH4 Rabbit pAb (CAB8303) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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